ICCAS OpenIR
Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces
Jing, Benxin1,2; Yang, Qingbo1; Zhu, Yingxi3; Zhao, Jiang1
2009-12-15
Source PublicationJOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS
ISSN0887-6266
Volume47Issue:24Pages:2541-2546
AbstractThe motion of DNA chain under electric field when it is in transient contact with the solid surfaces in aqueous Solution was studied by single molecule fluorescence microscopy at the total internal reflection geometry (TIRFM). In situ observation discovered that single lambda-DNA chains driven by electric field made transient contact with the solid sur-face and made hitting-sliding-leaving-like motion along the surface. By varying the surface chemistry from the negative-charged silanol group-rich surface to positive-charged amino group-rich surface, as well as hydrophobic surfaces, the dependence of DNA mobility on the surface-DNA inter-action was studied. The results show that a dependence of the mobility of DNA oil the surface polarity with respect to DNA itself. The study on different surfaces rich of silanol, amide, amino, and methyl groups show a sequence of DNA mobility of silanol > amide > amino. The mobility of DNA on methyl terminated surface was found to be similar to that on amino surface. (C) 2009 Wiley Periodicals, Inc J Polym Sci Part B Polym Phys 47 2541-2546, 2009
KeywordFluorescence Interfaces Polyelectrolytes
DOI10.1002/polb.21880
Indexed BySCI ; ISTP
Language英语
WOS IDWOS:000272561100013
PublisherJOHN WILEY & SONS INC
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/66388
Collection中国科学院化学研究所
Corresponding AuthorZhao, Jiang
Affiliation1.Chinese Acad Sci, Joint Lab Polymer Sci & Mat, State Key Lab Polymer Phys & Chem, Beijing Natl Lab Mol Sci,Inst Chem, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China
3.Univ Notre Dame, Dept Chem & Biomol Engn, Notre Dame, IN 46556 USA
Recommended Citation
GB/T 7714
Jing, Benxin,Yang, Qingbo,Zhu, Yingxi,et al. Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces[J]. JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS,2009,47(24):2541-2546.
APA Jing, Benxin,Yang, Qingbo,Zhu, Yingxi,&Zhao, Jiang.(2009).Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces.JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS,47(24),2541-2546.
MLA Jing, Benxin,et al."Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces".JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS 47.24(2009):2541-2546.
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