ICCAS OpenIR
PLLA-PCys co-electrospun fibers for capture and elution of glutathione S-transferase
Lu TianCheng1,2,3; Sun Jing1,3; Dong XiaoQing1,3; Chen XueSi1; Wang Yu1; Jing XiaBin1
2009-12-01
Source PublicationSCIENCE IN CHINA SERIES B-CHEMISTRY
ISSN1006-9291
Volume52Issue:12Pages:2033-2037
AbstractThe copolymer poly(L-lactic acid)-b-poly(L-cysteine) (PLA-b-PCys) was co-electrospun with PLGA into ultrafine fibers. The reduced glutathione (GSH) was conjugated to the fiber surfaces via disulfide bonds. The glutathione S-transferase (GST) was captured onto the GSH fibers via specific substrate-enzyme interaction between the bound GSH and GST. The captured GST was eluted with free GSH aqueous solution and lyophilized to get pure GST powders. The results show that the GSH moieties on the fiber surface retain the bioactivity of the free GSH and thus they can bind specifically with GST and the GST in solution is captured onto the fiber surface. In addition, the bound GSH is not as active as free GSH so that the captured GST can be eluted off from the fiber by free GSH aqueous solution. Based on this principle, GST itself or its fused proteins can be separated and purified very easily. The preliminary purification efficiency is 6.5 mg center dot(g(PCys))(-1). Further improvements are undertaken.
KeywordElectrospun Fibers Glutathione Glutathione S-transferase Protein Separation And Purification
DOI10.1007/s11426-009-0291-9
Indexed BySCI
Language英语
WOS IDWOS:000272917200001
PublisherSCIENCE PRESS
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/66336
Collection中国科学院化学研究所
Corresponding AuthorJing XiaBin
Affiliation1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
2.Jilin Agr Univ, Inst Life Sci, Changchun 130118, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Lu TianCheng,Sun Jing,Dong XiaoQing,et al. PLLA-PCys co-electrospun fibers for capture and elution of glutathione S-transferase[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(12):2033-2037.
APA Lu TianCheng,Sun Jing,Dong XiaoQing,Chen XueSi,Wang Yu,&Jing XiaBin.(2009).PLLA-PCys co-electrospun fibers for capture and elution of glutathione S-transferase.SCIENCE IN CHINA SERIES B-CHEMISTRY,52(12),2033-2037.
MLA Lu TianCheng,et al."PLLA-PCys co-electrospun fibers for capture and elution of glutathione S-transferase".SCIENCE IN CHINA SERIES B-CHEMISTRY 52.12(2009):2033-2037.
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