ICCAS OpenIR
Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy
Cao, Rui-Guo; Zhu, Bin; Li, Jingjian; Xu, Dongsheng
2009-09-01
Source PublicationELECTROCHEMISTRY COMMUNICATIONS
ISSN1388-2481
Volume11Issue:9Pages:1815-1818
AbstractA novel electrochemical biosensor with high sensitivity and selectivity for mercuric ion detection, based on DNA self-assembly electrode, is designed. Thiol functionalized poly-T oligonucleotides were used as gold electrode modifier through formation of Au-S bond between DNA and gold electrode. In presence of Hg(2+) ions, the specific coordination between Hg(2+) and thymine bases can change parallel ss-DNA from linear to hairpin structures, which can cause the release of partial DNA molecules from the surface of the electrode. The density of DNA on the surface of electrode correlated with the concentration of mercury in the solution and can be monitored by electrochemical impedance spectroscopy. The limit of detection of this method is pM level of mercuric ions which is far below the upper limit of Hg(2+) mandated by United States Environmental Protection Agency (EPA), 2 ppb (10 nM). In addition, this method showed excellent selectivity. A series of divalent metal ions, including Ni(2+), Co(2+), Mg(2+), Zn(2+), Ba(2+) and Cd(2+), have little interference with the detection of Hg(2+). (C) 2009 Elsevier B.V. All rights reserved.
KeywordLable-free Dna Biosensor Mercury Electrochemical Impedance Spectroscopy
DOI10.1016/j.elecom.2009.07.029
Indexed BySCI
Language英语
WOS IDWOS:000270347500023
PublisherELSEVIER SCIENCE INC
Citation statistics
Cited Times:54[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/66314
Collection中国科学院化学研究所
Corresponding AuthorLi, Jingjian
AffiliationPeking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Cao, Rui-Guo,Zhu, Bin,Li, Jingjian,et al. Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy[J]. ELECTROCHEMISTRY COMMUNICATIONS,2009,11(9):1815-1818.
APA Cao, Rui-Guo,Zhu, Bin,Li, Jingjian,&Xu, Dongsheng.(2009).Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy.ELECTROCHEMISTRY COMMUNICATIONS,11(9),1815-1818.
MLA Cao, Rui-Guo,et al."Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy".ELECTROCHEMISTRY COMMUNICATIONS 11.9(2009):1815-1818.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Cao, Rui-Guo]'s Articles
[Zhu, Bin]'s Articles
[Li, Jingjian]'s Articles
Baidu academic
Similar articles in Baidu academic
[Cao, Rui-Guo]'s Articles
[Zhu, Bin]'s Articles
[Li, Jingjian]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Cao, Rui-Guo]'s Articles
[Zhu, Bin]'s Articles
[Li, Jingjian]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.