ICCAS OpenIR
A New Light-Harvesting Conjugated Polyelectrolyte Microgel for DNA and Enzyme Detections
Feng, Xuli; Xu, Qingling; Liu, Libing; Wang, Shu
2009-12-15
Source PublicationLANGMUIR
ISSN0743-7463
Volume25Issue:24Pages:13737-13741
AbstractA new fluorescent microgel containing the CP moiety (PFP-NIPAm) was prepared with the size within 100 nm. Covalent linking of the Conjugated polyelectrolyte moiety into the microgel prevents leakage of the fluorophore while keeping its fluorescence property. The new fluorescent microgel call be used as all optical platform to detect DNA and enzyme. More importantly, because the electrostatic attraction dominates the interactions between cationic PFP-NIPAm microgel and negatively charged target, washing With high ionic strength aqueous solution call block their interactions and displace the target from the PFP-NIPAm microgel. Thus, PFP-NIPAm can be readily reusable for detection. Another unique feature is that the PFP-NIPAm microgel extends the detection media from homogeneous solution to solid phase, which shows great potential for biodetection in the real world using conjugated polymers. Furthermore, the detection call be carried out under UV light, and no expensive detection instrumentation is needed. In principle, such all assay system Could be expanded to a high-throughput assay.
DOI10.1021/la901444c
Indexed BySCI
Language英语
WOS IDWOS:000272394400007
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/66164
Collection中国科学院化学研究所
Corresponding AuthorWang, Shu
AffiliationChinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Feng, Xuli,Xu, Qingling,Liu, Libing,et al. A New Light-Harvesting Conjugated Polyelectrolyte Microgel for DNA and Enzyme Detections[J]. LANGMUIR,2009,25(24):13737-13741.
APA Feng, Xuli,Xu, Qingling,Liu, Libing,&Wang, Shu.(2009).A New Light-Harvesting Conjugated Polyelectrolyte Microgel for DNA and Enzyme Detections.LANGMUIR,25(24),13737-13741.
MLA Feng, Xuli,et al."A New Light-Harvesting Conjugated Polyelectrolyte Microgel for DNA and Enzyme Detections".LANGMUIR 25.24(2009):13737-13741.
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