ICCAS OpenIR
NMR studies on binding sites and aggregation-disassociation of fluorinated surfactant sodium perfluorooctanoate on protein ubiquitin
Lu, Run-Chao1; Guo, Xian-Rong1,2; Jin, Changwen1,2; Xiao, Jin-Xin1,3
2009-02-01
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
ISSN0304-4165
Volume1790Issue:2Pages:134-140
AbstractThe fluorinated surfactant sodium perfluorooctanoate (SPFO) Could bind onto ubiquitin (UBQ) and induce the unfolding of UBQ By using (15)N-edited heteronuclear single-quantum coherence (HSQC) NMR and (19)F NMR to monitor (15)N-labeled UBQ and SPFO, respectively, the binding sites and the aggregation process of SPFO on UBQ at various SPFO concentrations were observed. A detailed process from specific binding to cooperative binding of SPFO oil UBQ, and a detailed structure change of UBQ upon the increase of SPFO concentration were obtained. The refolding of UBQ in UBQ-SPFO complex was carried out by adding cationic surfactant. It was shown that added cationic surfactants formed mixed micelles with SPFO and resulted in the dissociation of the UBQ-SPFO complex, and consequently, most ubiquitin could be refolded to its native state. (C) 2008 Elsevier B.V. All rights reserved.
KeywordUbiquitin Sodium Perfluorooctanoate Dodecyltrimethylammonium Chloride Unfolding Binding Sites
DOI10.1016/j.bbagen.2008.10.009
Indexed BySCI
Language英语
WOS IDWOS:000262744100007
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/66162
Collection中国科学院化学研究所
Corresponding AuthorXiao, Jin-Xin
Affiliation1.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Beijing Nucl Magnet Resonance Ctr, Beijing 100871, Peoples R China
3.Beijing FLUOBON Surfactant Inst, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Lu, Run-Chao,Guo, Xian-Rong,Jin, Changwen,et al. NMR studies on binding sites and aggregation-disassociation of fluorinated surfactant sodium perfluorooctanoate on protein ubiquitin[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2009,1790(2):134-140.
APA Lu, Run-Chao,Guo, Xian-Rong,Jin, Changwen,&Xiao, Jin-Xin.(2009).NMR studies on binding sites and aggregation-disassociation of fluorinated surfactant sodium perfluorooctanoate on protein ubiquitin.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1790(2),134-140.
MLA Lu, Run-Chao,et al."NMR studies on binding sites and aggregation-disassociation of fluorinated surfactant sodium perfluorooctanoate on protein ubiquitin".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1790.2(2009):134-140.
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