ICCAS OpenIR
Label-Free, Homogeneous, and Fluorescence "Turn-On" Detection of Protease Using Conjugated Polyelectrolytes
An, Lingling; Liu, Libing; Wang, Shu
2009-02-01
Source PublicationBIOMACROMOLECULES
ISSN1525-7797
Volume10Issue:2Pages:454-457
AbstractA label-free, homogeneous, and sensitive fluorescence "turn-on" approach is designed to rapidly detect protease using serine functionalized polythiophene (POWT). The fluorescence of POWT solution containing bovine serum albumin (BSA) as substrate is efficiently quenched by Cu(2+) ions through coordinate interaction with serine moieties. Upon adding trypsin to the solution, the BSA is cleaved into amino acid or peptide fragments, which are stronger Cu(2+) chelators to form more stable complexes with Cu(2+) ions. Thus, the Cu(2+) ion is displaced from POWT and the fluorescence of POWT is recovered. By triggering the "turn-on" signal of POWT, it is possible to detect the trypsin in real time. "Turn-on" response as readout signal is able to effectively reduce background noise and increase detection sensitivity. Because of the simplicity, high sensitivity, and rapid response, our new enzyme assay shows great potential for protease detection in the future.
DOI10.1021/bm801036h
Indexed BySCI
Language英语
WOS IDWOS:000263226300033
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:46[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/66120
Collection中国科学院化学研究所
Corresponding AuthorWang, Shu
AffiliationChinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
An, Lingling,Liu, Libing,Wang, Shu. Label-Free, Homogeneous, and Fluorescence "Turn-On" Detection of Protease Using Conjugated Polyelectrolytes[J]. BIOMACROMOLECULES,2009,10(2):454-457.
APA An, Lingling,Liu, Libing,&Wang, Shu.(2009).Label-Free, Homogeneous, and Fluorescence "Turn-On" Detection of Protease Using Conjugated Polyelectrolytes.BIOMACROMOLECULES,10(2),454-457.
MLA An, Lingling,et al."Label-Free, Homogeneous, and Fluorescence "Turn-On" Detection of Protease Using Conjugated Polyelectrolytes".BIOMACROMOLECULES 10.2(2009):454-457.
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