ICCAS OpenIR
Effects of Quantum Dots in Polymerase Chain Reaction
Wang, Libing1,2; Zhu, Yingyue1; Jiang, Yuan3; Qiao, Ruirui4; Zhu, Shuifang5; Chen, Wei1; Xu, Chuanlai1
2009-05-28
Source PublicationJOURNAL OF PHYSICAL CHEMISTRY B
ISSN1520-6106
Volume113Issue:21Pages:7637-7641
AbstractThe effects of quantum dots (QDs) on the elimination of nonspecific amplification of the polymerase chain reaction (PCR) were investigated. It was found that QDs could increase the specificity of the PCR at different annealing temperatures and with DNA templates of different lengths. The effects of QDs on the efficiency of the PCR were also studied, and the results showed that there was no enhancement. The mechanisms underlying, these effects are discussed. This method could be used to modify the amplification results of the conventional PCR. Furthermore, this technology could make the PCR more widely applicable, especially in the multi-PCR reaction system with different annealing temperatures. This is of great significance for gene diagnosis.
DOI10.1021/jp902404y
Indexed BySCI
Language英语
WOS IDWOS:000266296700031
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:42[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/66058
Collection中国科学院化学研究所
Corresponding AuthorChen, Wei
Affiliation1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
2.Hunan Import & Export Inspect & Quarantine Bur, Changsha 410000, Hunan, Peoples R China
3.Jiangsu Import & Export Inspect & Quarantine Bur, Food Lab, Nanjing 210000, Jiangsu, Peoples R China
4.Chinese Acad Sci, Inst Chem, Beijing 100081, Peoples R China
5.Chinese Acad Inspect & Quarantine, Beijing 100082, Peoples R China
Recommended Citation
GB/T 7714
Wang, Libing,Zhu, Yingyue,Jiang, Yuan,et al. Effects of Quantum Dots in Polymerase Chain Reaction[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2009,113(21):7637-7641.
APA Wang, Libing.,Zhu, Yingyue.,Jiang, Yuan.,Qiao, Ruirui.,Zhu, Shuifang.,...&Xu, Chuanlai.(2009).Effects of Quantum Dots in Polymerase Chain Reaction.JOURNAL OF PHYSICAL CHEMISTRY B,113(21),7637-7641.
MLA Wang, Libing,et al."Effects of Quantum Dots in Polymerase Chain Reaction".JOURNAL OF PHYSICAL CHEMISTRY B 113.21(2009):7637-7641.
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