ICCAS OpenIR
Simultaneous and sensitive determination of multiplex chemical residues based on multicolor quantum dot probes
Peng, Chifang1; Li, Zhuokun1; Zhu, Yinyue1; Chen, Wei1; Yuan, Yuan1; Liu, Liqiang1; Li, Qiusheng1; Xu, Dinghua1; Qiao, Ruirui2; Wang, Libing1; Zhu, Shuifang1; Jin, Zhengyu1; Xu, Chuanlai1
2009-08-15
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume24Issue:12Pages:3657-3662
AbstractBiotinylated denatured bovine serum albumin (Bt-dBSA)-coated cadmium telluride (CdTe) quantum dot (QD) conjugates were prepared and used to develop the multiplexed fluoroimmunoassay for the simultaneous determination of five chemical residues. An immune complex was formed using avidin as the bridge to link the Bt-dBSA-QDs with the antibodies. Primarily, individual quantitative determinations of five representative chemical residues were carried out based on the different emission properties of the QDs. Five antibodies were then conjugated with the corresponding QDs to establish the indirect competition fluorescent-linked immunosorbent assay (ic-FLISA) for the simultaneous detection of five chemicals in one well of a microplate. The linear range for dexamethason (DEX) was from 0.33 mu g/kg to 10 mu g/kg, 0.28 mu g/kg to 10 mu g/kg for gentamicin (GM), 0.16 mu g/kg to 25 mu g/kg for clonazepam (UP), 0.17 mu g/kg to 10 mu g/kg for medroxyprogesterone acetate (MPA) and 0.32 mu g/kg to 25 mu g/kg for ceftiofur (CEF), respectively. The limit of detection (LOD) for the simultaneous determination of DEX, GM, CZP, MPA and CEF were as low as 0.13 mu g/kg, 0.16 mu g/kg, 0.07 mu g/kg, 0.06 mu g/kg and 0.14 mu g/kg, respectively. This detection method was used to analyze samples of pork muscle and the recoveries ranged from 61.3% to 80.3% for DEX and from 74.0% to 87.2% for MPA. Further more, good correlation between the novel ic-FLISA and traditional ELISA was demonstrated during the determination of DEX and MPA residues in real samples. The QD-based protocol described here is less time consuming than the classical method and it may be sufficiently flexible to be used in other systems for the simultaneous multicolor detection of the drugs. (C) 2009 Elsevier B.V. All rights reserved.
KeywordSimultaneous Sensitive Determination Multiplex Chemical Residues Multicolor Quantum Dot Probes
DOI10.1016/j.bios.2009.05.031
Indexed BySCI
Language英语
WOS IDWOS:000269278300040
PublisherELSEVIER ADVANCED TECHNOLOGY
Citation statistics
Cited Times:77[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65992
Collection中国科学院化学研究所
Corresponding AuthorJin, Zhengyu
Affiliation1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu Prov, Peoples R China
2.CAS, Inst Chem, Beijing 10008, Peoples R China
Recommended Citation
GB/T 7714
Peng, Chifang,Li, Zhuokun,Zhu, Yinyue,et al. Simultaneous and sensitive determination of multiplex chemical residues based on multicolor quantum dot probes[J]. BIOSENSORS & BIOELECTRONICS,2009,24(12):3657-3662.
APA Peng, Chifang.,Li, Zhuokun.,Zhu, Yinyue.,Chen, Wei.,Yuan, Yuan.,...&Xu, Chuanlai.(2009).Simultaneous and sensitive determination of multiplex chemical residues based on multicolor quantum dot probes.BIOSENSORS & BIOELECTRONICS,24(12),3657-3662.
MLA Peng, Chifang,et al."Simultaneous and sensitive determination of multiplex chemical residues based on multicolor quantum dot probes".BIOSENSORS & BIOELECTRONICS 24.12(2009):3657-3662.
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