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EFFECT OF OVERLAP CONCENTRATION AND PERSISTENCE LENGTH ON DNA SEPARATION IN POLYMER SOLUTIONS BY ELECTROPHORESIS
Ke, Fu-you; Mo, Xiu-lei; Liang, De-hai1
2009-09-01
Source PublicationCHINESE JOURNAL OF POLYMER SCIENCE
ISSN0256-7679
Volume27Issue:5Pages:601-610
AbstractThe persistence length and the overlapconcentration (c*) of poly(ethylene oxide) (PEO) and hydroxyethylcellulose (HEC) with similar molecular weight in 1 x TBE buffer were studied by laser light scattering and viscometry. Their effect on DNA separation was investigated by capillary electrophoresis. It was determined that the persistence length of HEC was at least 5 times higher than that of PEO. Therefore, the c* of HEC was smaller than that of PEO by a factor of ca. 2.5. It was also found that the c* values determined by laser light scattering were much lower than those by viscometry. We attributed it to the state of polymer chains in which c* was measured: laser light scattering determined the c* in ultradilute solution where the polymer chains were in the static state; while viscometry determined the c* in dilute or semidilute concentration region with polymer chains being in the hydrodynamic flowing state. When used in DNA separation, polymer chains were in different states due to the disturbance of DNA molecules, making c* region dependent. When the real concentration was normalized by c*, PEO and HEC showed almost equal performance in separating Phi X174/Hae III DNA digest in dilute solution, even though their persistence lengths were quite different.
KeywordPersistence Length Overlap Concentration Polymer Matrix Dna Separation Electrophoresis
DOI10.1142/S0256767909004291
Indexed BySCI
Language英语
WOS IDWOS:000269451500001
PublisherWORLD SCIENTIFIC PUBL CO PTE LTD
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65956
Collection中国科学院化学研究所
Corresponding AuthorLiang, De-hai
Affiliation1.Peking Univ, Coll Chem & Mol Engn, Dept Polymer Sci & Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
2.Peking Univ, Key Lab Polymer Chem & Phys, Minist Educ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Ke, Fu-you,Mo, Xiu-lei,Liang, De-hai. EFFECT OF OVERLAP CONCENTRATION AND PERSISTENCE LENGTH ON DNA SEPARATION IN POLYMER SOLUTIONS BY ELECTROPHORESIS[J]. CHINESE JOURNAL OF POLYMER SCIENCE,2009,27(5):601-610.
APA Ke, Fu-you,Mo, Xiu-lei,&Liang, De-hai.(2009).EFFECT OF OVERLAP CONCENTRATION AND PERSISTENCE LENGTH ON DNA SEPARATION IN POLYMER SOLUTIONS BY ELECTROPHORESIS.CHINESE JOURNAL OF POLYMER SCIENCE,27(5),601-610.
MLA Ke, Fu-you,et al."EFFECT OF OVERLAP CONCENTRATION AND PERSISTENCE LENGTH ON DNA SEPARATION IN POLYMER SOLUTIONS BY ELECTROPHORESIS".CHINESE JOURNAL OF POLYMER SCIENCE 27.5(2009):601-610.
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