ICCAS OpenIR
On-column labeling technique and chiral ligand-exchange CE with zinc(II)-L-arginine complex as a chiral selector for assay of dansylated D,L-amino acids
Qi, Li1,2,3; Yang, Gengliang1
2009-08-01
Source PublicationELECTROPHORESIS
ISSN0173-0835
Volume30Issue:16Pages:2882-2889
AbstractA novel on-column labeling method of amino acid (AA) enantiomers by using dansyl chloride (Dns-Cl) has been explored combined with chiral ligand-exchange CE (CLE-CE) technique and UV detection. Efficient labeling was achieved by sequential injection of buffer, Dns-Cl, AA enantiomers, Dns-Cl and buffer at 0.2 psi for 10.0, 3.0, 24.0, 3.0, and 10.0 s, respectively. After injection, the sandwich sections were allowed to react at room temperature for 35.0 min. With this procedure, successful on-column labeling and CLE-CE separation of 17 pairs AA enantiomers have been achieved with a buffer of 100.0 mM boric acid, 5.0 mM ammonium acetate, 3.0 mM ZnSO(4) and 6.0 MM L-Arg at pH 8.4, giving nine pairs fully enantioresolved with resolution in between 2.0 and 5.1. CLE-CE of some individual and mixed pairs was also demonstrated, much the same as using pre-column labeling. As validated by both artificially prepared solutions and serum samples, this new method was shown to be applicable to the quantitative analysis, with a linear range between 14.0 mu M and 3.7 mM, correlation coefficient above 0.99 and recovery in between 90.4% and 111.7%. It was also demonstrated that the migration time-temperature based curve allows for temperature determination in CE by using this new method.
KeywordDansyl Amino Acid Enantioseparation Ligand-exchange Ce On-column Labeling Zinc Complex
DOI10.1002/elps.200800753
Indexed BySCI
Language英语
WOS IDWOS:000269712500015
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65800
Collection中国科学院化学研究所
Corresponding AuthorYang, Gengliang
Affiliation1.Hebei Univ, Coll Pharmaceut Sci, Baoding 071002, Peoples R China
2.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing 100080, Peoples R China
3.Beijing Natl Lab Mol Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Qi, Li,Yang, Gengliang. On-column labeling technique and chiral ligand-exchange CE with zinc(II)-L-arginine complex as a chiral selector for assay of dansylated D,L-amino acids[J]. ELECTROPHORESIS,2009,30(16):2882-2889.
APA Qi, Li,&Yang, Gengliang.(2009).On-column labeling technique and chiral ligand-exchange CE with zinc(II)-L-arginine complex as a chiral selector for assay of dansylated D,L-amino acids.ELECTROPHORESIS,30(16),2882-2889.
MLA Qi, Li,et al."On-column labeling technique and chiral ligand-exchange CE with zinc(II)-L-arginine complex as a chiral selector for assay of dansylated D,L-amino acids".ELECTROPHORESIS 30.16(2009):2882-2889.
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