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Design, synthesis and screening of antisense peptide based combinatorial peptide libraries towards an aromatic region of SARS-CoV
Huang, Yanyan; Zhao, Rui; Luo, Jia; Xiong, Shaoxiang; Shangguan, Dihua; Zhang, Hangwu; Liu, Guoquan; Chen, Yi
2008-03-01
Source PublicationJOURNAL OF MOLECULAR RECOGNITION
ISSN0952-3499
Volume21Issue:2Pages:122-131
AbstractA combination of high-performance affinity chromatography and antisense peptide based combinatorial peptide libraries was used to screen a potential inhibitor for SARS-CoV. An aromatic-amino acid-rich region within the transmembrane domain at the C terminal of spike (S) protein identified as a membrane-active region was chosen as the target sense peptide (SP) and immobilized as affinity ligand. Four antisense peptides were designed based on the degeneracy of genetic codes. One of them was screened as the lead peptide to construct the extended peptide libraries (EPL). The library screening was carried out at pH 5.5 so as to mimic the low-pH milieu required by virus fusion. After five cycles of screening, a cloclecapeptide KKKKYRNIRRPG (DP) was identified to possess the highest binding affinity to the immobilized sense peptide. The dissociation constant of the complex between the DP and the SP was 5.64 x 10(-7) M in a physiological condition. The recognition between the DP and recombinant SARS S protein was demonstrated by ELISA assay to be in a saturable way. The competitive inhibition of the sense peptide in the competitive ELISA reveals the affinity binding between the DIP and SARS S protein is specific and directed towards the target SP of the S protein. The results indicate this preferred polypeptide can be used as a lead compound of potent inhibitor of SARS-CoV. The mechanism study suggests the specific recognition between the DIP and the target peptide was due to sequence-dependent and multi-modal affinity interaction. Copyright (c) 2008 John Wiley & Sons, Ltd.
KeywordCombinatorial Chemistry High-performance Affinity Chromatography Antisense Peptides Potential Inhibitor Sars-cov
DOI10.1002/jmr.880
Indexed BySCI
Language英语
WOS IDWOS:000255105800006
PublisherJOHN WILEY & SONS LTD
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65732
Collection中国科学院化学研究所
Corresponding AuthorZhao, Rui
AffiliationChinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Huang, Yanyan,Zhao, Rui,Luo, Jia,et al. Design, synthesis and screening of antisense peptide based combinatorial peptide libraries towards an aromatic region of SARS-CoV[J]. JOURNAL OF MOLECULAR RECOGNITION,2008,21(2):122-131.
APA Huang, Yanyan.,Zhao, Rui.,Luo, Jia.,Xiong, Shaoxiang.,Shangguan, Dihua.,...&Chen, Yi.(2008).Design, synthesis and screening of antisense peptide based combinatorial peptide libraries towards an aromatic region of SARS-CoV.JOURNAL OF MOLECULAR RECOGNITION,21(2),122-131.
MLA Huang, Yanyan,et al."Design, synthesis and screening of antisense peptide based combinatorial peptide libraries towards an aromatic region of SARS-CoV".JOURNAL OF MOLECULAR RECOGNITION 21.2(2008):122-131.
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