ICCAS OpenIR
Aptamer-based electrochemical sensors that are not based on the target binding-induced conformational change of aptamers
Lu, Ying; Zhu, Ningning; Yu, Ping; Mao, Lanqun
2008-09-01
Source PublicationANALYST
ISSN0003-2654
Volume133Issue:9Pages:1256-1260
AbstractThis study describes a new kind of aptamer-based electrochemical sensor that is not based on the target binding-induced conformational change of the aptamers by using a 15-mer thrombin-binding aptamer (5'-GGTTGGTGTGGTTGG-3') as the model oligonucleotide. The sensors are developed by first self-assembling the aptamer (i.e. a thrombin-binding aptamer) onto an An electrode and then hybridizing the assembled aptamer with a ferrocene (Fc)-labeled short aptamer-complementary DNA oligonucleotide to form an electroactive double-stranded DNA (ds-DNA) oligonucleotide onto the Au electrode. The binding of the target (i.e. thrombin) towards the aptamer essentially destroys the Watson-Crick helix structure of the ds-DNA oligonucleotide assembled onto the electrode and leads to the dissociation of the Fe-labeled short complementary DNA oligonucleotide from the electrode surface to the solution, resulting in a decrease in the current signal obtained at the electrode, which can be used for the determination of the target. With the thrombin-binding aptamer as the model oligonucleotide, the current decrease obtained with the aptamer-based electrochemical sensors is linear with the concentration of thrombin within the concentration range from 0 to 10 nM (Delta I/nA = 6.7C(thrombin)/nM + 2.8, gamma = 0.975). Unlike most kinds of existing aptamer-based electrochemical sensor, the electrochemical aptasensors demonstrated here are not based on the conformational change of the aptamers induced by the specific target binding. Moreover, the aptasensors are essentially label-free and are very responsive toward the targets. This study may pave a facile and general way to the development of aptamer-based electrochemical sensors.
DOI10.1039/b807913g
Indexed BySCI
Language英语
WOS IDWOS:000259449400021
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:46[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65658
Collection中国科学院化学研究所
Corresponding AuthorMao, Lanqun
AffiliationChinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Lu, Ying,Zhu, Ningning,Yu, Ping,et al. Aptamer-based electrochemical sensors that are not based on the target binding-induced conformational change of aptamers[J]. ANALYST,2008,133(9):1256-1260.
APA Lu, Ying,Zhu, Ningning,Yu, Ping,&Mao, Lanqun.(2008).Aptamer-based electrochemical sensors that are not based on the target binding-induced conformational change of aptamers.ANALYST,133(9),1256-1260.
MLA Lu, Ying,et al."Aptamer-based electrochemical sensors that are not based on the target binding-induced conformational change of aptamers".ANALYST 133.9(2008):1256-1260.
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