ICCAS OpenIR
Antigen synthetic strategy and immunoassay development for detection of acrylamide in foods
Zhou, Shuang; Zhang, Chen; Wang, Dan; Zhao, Meiping
2008
Source PublicationANALYST
ISSN0003-2654
Volume133Issue:7Pages:903-909
AbstractAcrylamide, a toxic and carcinogenic compound, has been found to be present in a range of processed starchy foods. To prepare an effective immunogen compound for acrylamide, N-acryloxysuccinimide (NAS) was conjugated to bovine serum albumin (BSA) at a high molar ratio of 21.2 : 1. Antisera were obtained by immunization of rabbits with additional booster injections of the NAS-BSA conjugate after the regular process. The IgGs purified by an ammonium sulfate precipitation method were further fractionated with a BSA-immobilized immunoaffinity column. The affinity constant between the collected antibody and coated antigen (NAS-ovalbumin) is found to be 6.7 x 10(7) L mol(-1). Asparagine, the key precursor of acrylamide formation in foods, showed negligible cross-reactivity to the antibody. A biotin-avidin enzyme-linked immunosorbent assay (BA-ELISA) was developed and the optimum assay medium was found to be 0.1 mol L(-1) NaHCO(3) (pH 8.3, containing 0.5 mol L(-1) NaCl). The BA-ELISA afforded a practical sensitivity with a working range of 10-100 000 ng mL(-1) and a detection limit of 6 ng mL(-1). The assay was applied to detect acrylamide in potato fries and biscuits and the quantitative results were in good agreement with those obtained by the high-performance liquid chromatography method. This immunoassay will be very useful for monitoring acrylamide in food samples.
DOI10.1039/b716526a
Indexed BySCI
Language英语
WOS IDWOS:000257125100012
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65628
Collection中国科学院化学研究所
Corresponding AuthorZhao, Meiping
AffiliationPeking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Shuang,Zhang, Chen,Wang, Dan,et al. Antigen synthetic strategy and immunoassay development for detection of acrylamide in foods[J]. ANALYST,2008,133(7):903-909.
APA Zhou, Shuang,Zhang, Chen,Wang, Dan,&Zhao, Meiping.(2008).Antigen synthetic strategy and immunoassay development for detection of acrylamide in foods.ANALYST,133(7),903-909.
MLA Zhou, Shuang,et al."Antigen synthetic strategy and immunoassay development for detection of acrylamide in foods".ANALYST 133.7(2008):903-909.
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