ICCAS OpenIR
Investigation on the interaction between a heterocyclic aminal derivative, SBDC, and human serum albumin
Zhou, Qiuju1,2; Xiang, Junfeng1; Tang, Yalin1; Liao, Jiangpeng2,3; Yu, Chuyi3; Zhang, Hong1; Li, Lin1; Yang, Yueyang1; Xu, Guangzhi1
2008-01-15
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume61Issue:1Pages:75-80
AbstractThe interaction between a novel promising drug (spiro[(2R,3R,4S)-4-benzyloxy-2,3-isopropylidene-dioxy-1-oxa-cyclopentane-5,5'-(2-benzoylmethylene-1,3-diaza-cyclohexane)] (SBDC)) and human serum albumin (HSA) under physiological conditions has been investigated by using fluorescence, absorption, and circular dichroism (CD) spectroscopic techniques in combination with protein-ligand docking study. It was observed that SBDC has a strong ability to quench the intrinsic fluorescence of HSA through a static quenching procedure. The association constants of SBDC with HSA were determined at different temperatures based on fluorescence quenching results. The negative Delta H and positive Delta S values in case of SBDC-HSA complex showed that apart from an initial hydrophobic association, both van der Waals interactions and hydrogen bonding play a vital role in the binding of SBDC to HSA. The quantitative analysis data of CD spectra showed that the binding of SBDC to HSA induced conformational changes in HSA and the alpha-helix of 52.1% in free HSA increased to 55.7% in HSA-SBDC complex. The distance between donor (HSA) and acceptor (SBDC) was obtained according to the Forster's theory of non-radiation energy transfer. Data obtained by spectroscopic techniques and protein-ligand docking study suggested that SBDC binds to residues located in subdomain IIA of HSA. (C) 2007 Elsevier B.V. All rights reserved.
KeywordHeterocyclic Ketene Aminals Human Serum Albumin Fluorescence Quenching Circular Dichroism Protein-ligand Docking
DOI10.1016/j.colsurfb.2007.07.007
Indexed BySCI
Language英语
WOS IDWOS:000252584300011
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65588
Collection中国科学院化学研究所
Corresponding AuthorTang, Yalin
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci BNLMS, Ctr Mol Sci,State Key Lab Struct Chem Unstable &, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
3.Chinese Acad Sci, Inst Chem, Lab Mol Recognit & Select Synth, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Qiuju,Xiang, Junfeng,Tang, Yalin,et al. Investigation on the interaction between a heterocyclic aminal derivative, SBDC, and human serum albumin[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2008,61(1):75-80.
APA Zhou, Qiuju.,Xiang, Junfeng.,Tang, Yalin.,Liao, Jiangpeng.,Yu, Chuyi.,...&Xu, Guangzhi.(2008).Investigation on the interaction between a heterocyclic aminal derivative, SBDC, and human serum albumin.COLLOIDS AND SURFACES B-BIOINTERFACES,61(1),75-80.
MLA Zhou, Qiuju,et al."Investigation on the interaction between a heterocyclic aminal derivative, SBDC, and human serum albumin".COLLOIDS AND SURFACES B-BIOINTERFACES 61.1(2008):75-80.
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