ICCAS OpenIR
The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges
Lu, Tiancheng1,2; Chen, Xuesi1; Shi, Quan1,2; Wang, Yu1; Zhang, Peibiao1; Jing, Xiabin1
2008-11-01
Source PublicationACTA BIOMATERIALIA
ISSN1742-7061
Volume4Issue:6Pages:1770-1777
AbstractThis paper aims at developing novel bioactive fibrous mats for protein immobilization and for protein separation/purification. For this purpose, an amphiphilic triblock copolymer, biotinylated poly(ethylene glycol)-b-poly(L-lactide)-b-poly(L-lysine) was co-electrospun together with poly(L-lactide-co-glycolide) into ultrafine fibers similar to 2 mu m in diameter, and a layer of blocking agent was coated on the fiber surfaces to block off possible non-specific binding of proteins. The biotin species retained their ability to specifically recognize and bind streptavidin, and the immobilized streptavidin could further combine with biotinylated antibodies, antigens and other biological moieties. Horseradish peroxidase-labeled streptavidin and fluorescein isothiocyanate-labeled goat globulin were used to detect the immobilizations of streptavidin and rabbit anti-goat IgG(H + L) via enzyme-linked immunoassay and confocal laser scanning microscope, respectively. The immobilized antigen was eluted from the fiber substrate with a glycine/HCl solution and the eluted antigen retained its bioactivity. Therefore, these biotin-carrying composite fibers have a variety of uses, including selective immobilization of functional proteins, antigen/antibody separation and purification, and vaccine preparation. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
KeywordElectrospun Fibers Protein Immobilization Immunoassay Antibodies Antigens
DOI10.1016/j.actbio.2008.05.006
Indexed BySCI
Language英语
WOS IDWOS:000261253400021
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65534
Collection中国科学院化学研究所
Corresponding AuthorJing, Xiabin
Affiliation1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Lu, Tiancheng,Chen, Xuesi,Shi, Quan,et al. The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges[J]. ACTA BIOMATERIALIA,2008,4(6):1770-1777.
APA Lu, Tiancheng,Chen, Xuesi,Shi, Quan,Wang, Yu,Zhang, Peibiao,&Jing, Xiabin.(2008).The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges.ACTA BIOMATERIALIA,4(6),1770-1777.
MLA Lu, Tiancheng,et al."The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges".ACTA BIOMATERIALIA 4.6(2008):1770-1777.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lu, Tiancheng]'s Articles
[Chen, Xuesi]'s Articles
[Shi, Quan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lu, Tiancheng]'s Articles
[Chen, Xuesi]'s Articles
[Shi, Quan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lu, Tiancheng]'s Articles
[Chen, Xuesi]'s Articles
[Shi, Quan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.