ICCAS OpenIR
Base-by-Base Dynamics in DNA Hybridization Probed by Fluorescence Correlation Spectroscopy
Chen, Xudong; Zhou, Yan; Qu, Peng; Zhao, Xin Sheng1
2008-12-17
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume130Issue:50Pages:16947-16952
AbstractThermodynamics and dynamics of DNA hybridization/dehybridization at a terminal of a DNA duplex are investigated using steady-state fluorescence and fluorescence correlation spectroscopy (FCS). We introduce two pairs of dyes with different characteristic distances in fluorescence resonance energy transfer (FRET) to investigate the same process. The phenomenal discrepancy in the experimental observations between our two FRET pairs is incompatible with the traditional two-state model. We propose a so-called stretched exponential zipper (SEZ) model to successfully analyze the experimental data, through which the fundamental behavior of base-by-base hybridization/dehybridization is revealed. The dynamic parameters of the activation energy of single base-pair reaction derived from the two FRET pairs are consistent. The enthalpy change and entropy change of single base-pair formation are in agreement with theoretical prediction.
DOI10.1021/ja804628x
Indexed BySCI
Language英语
WOS IDWOS:000263320400032
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65388
Collection中国科学院化学研究所
Corresponding AuthorZhao, Xin Sheng
Affiliation1.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Peking Univ, Dept Biol Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Chen, Xudong,Zhou, Yan,Qu, Peng,et al. Base-by-Base Dynamics in DNA Hybridization Probed by Fluorescence Correlation Spectroscopy[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2008,130(50):16947-16952.
APA Chen, Xudong,Zhou, Yan,Qu, Peng,&Zhao, Xin Sheng.(2008).Base-by-Base Dynamics in DNA Hybridization Probed by Fluorescence Correlation Spectroscopy.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,130(50),16947-16952.
MLA Chen, Xudong,et al."Base-by-Base Dynamics in DNA Hybridization Probed by Fluorescence Correlation Spectroscopy".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 130.50(2008):16947-16952.
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