ICCAS OpenIR
Interactions between protein and porphyrin-containing cyclodextrin supramolecular system: a fluorescent sensing approach for albumin
Long, Liping1,3; Jin, Man Yu1; Zhang, Ying1; Yang, Ronghua1,2; Wang, Keming2
2008-09-01
Source PublicationANALYST
ISSN0003-2654
Volume133Issue:9Pages:1201-1208
AbstractThe interactions of meso-tetraphenylporphyrin (TPP), meso-tetraphenylporphyrin cobalt(II) (CoTPP) and protein in the presence of a cyclodextrin derivative, heptakis(2,6-di-O-n-octyl)-beta-cyclodextrin (Oc-beta-CD), have been investigated. In the presence of Oc-beta-CD, significant increase of TPP fluorescence was realized, but the increased fluorescence was quenched by CoTPP. To further investigate the fluorescence-quenched system and explore its potential application in bioanalysis, a strategy has been devised to restore the quenching fluorescence of TPP upon interacting with protein. The restoration of TPP fluorescence in the present system is fast and accomplished upon interaction with bovine serum albumin (BSA) or human serum albumin (HSA). On the basis of the spectroscopic measurement and excited state fluorescence lifetime, the mechanism of TPP fluorescence quenching is attributed to formation of a ground-state complex of TPP and CoTPP, and the fluorescence restoration is attributed to the binding of CoTPP with the protein molecule which destroys the aggregate, releasing the free base porphyrin. With optimized conditions, the calibration equations are linear from 0.80 to 75.4 mu g mL(-1) BSA and from 3.20 to 93.2 mu g mL(-1) HSA. The corresponding detection limits are 0.32 mu g mL(-1) for BSA and 1.06 mu g mL(-1) for HSA, respectively The method was used for the direct assay of HSA content in human serum. The result is comparable to that obtained by another method. The recovery from BSA in synthetic sample is also satisfactory.
DOI10.1039/b808754g
Indexed BySCI
Language英语
WOS IDWOS:000259449400012
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65326
Collection中国科学院化学研究所
Corresponding AuthorYang, Ronghua
Affiliation1.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China
3.Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413500, Hunan, Peoples R China
Recommended Citation
GB/T 7714
Long, Liping,Jin, Man Yu,Zhang, Ying,et al. Interactions between protein and porphyrin-containing cyclodextrin supramolecular system: a fluorescent sensing approach for albumin[J]. ANALYST,2008,133(9):1201-1208.
APA Long, Liping,Jin, Man Yu,Zhang, Ying,Yang, Ronghua,&Wang, Keming.(2008).Interactions between protein and porphyrin-containing cyclodextrin supramolecular system: a fluorescent sensing approach for albumin.ANALYST,133(9),1201-1208.
MLA Long, Liping,et al."Interactions between protein and porphyrin-containing cyclodextrin supramolecular system: a fluorescent sensing approach for albumin".ANALYST 133.9(2008):1201-1208.
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