ICCAS OpenIR
Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis
Deng, Wei; Cao, Aoneng; Lai, Luhua
2008-06-01
Source PublicationPROTEIN SCIENCE
ISSN0961-8368
Volume17Issue:6Pages:1102-1105
AbstractThe recently published microcrystal structures of amyloid fibrils from small peptides greatly enhanced our understanding of the atomic-level structure of the amyloid fibril. However, only a few amyloid fibrils can form microcrystals. The dansyl-tryptophan fluorescence resonance energy transfer (FRET) pair was shown to be able to detect the inter-peptide arrangement of the Transthyretin (105-115) amyloid fibril. In this study, we combined the known microcrystal structures with the corresponding FRET efficiencies to build a model for amyloid fibril structure classification. We found that fibrils with an antiparallel structural arrangement gave the largest FRET signal, those with a parallel arrangement gave the lowest FRET signal, and those with a mixed arrangement gave a moderate FRET signal. This confirms that the amyloid fibril structure patterns can be classified based on the FRET efficiency.
KeywordAmyloid Fibril Fret Classification Cross-beta Structure Elucidation
DOI10.1110/ps.083475108
Indexed BySCI
Language英语
WOS IDWOS:000256166600015
PublisherCOLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65298
Collection中国科学院化学研究所
Corresponding AuthorLai, Luhua
AffiliationPeking Univ, Beijing Natl Lab Mol Sci, State Ket Lab Struct Chem Unstable & Stable Speci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Deng, Wei,Cao, Aoneng,Lai, Luhua. Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis[J]. PROTEIN SCIENCE,2008,17(6):1102-1105.
APA Deng, Wei,Cao, Aoneng,&Lai, Luhua.(2008).Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis.PROTEIN SCIENCE,17(6),1102-1105.
MLA Deng, Wei,et al."Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis".PROTEIN SCIENCE 17.6(2008):1102-1105.
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