ICCAS OpenIR
Study of interaction between Smad7 and DNA by single-molecule force spectroscopy
Shi, Xiaoli1; Chen, Feng2; Yu, Junping1; Xu, Yongchun1; Zhang, Suping2; Chen, Ye-Guang2; Fang, Xiaohong1
2008-12-26
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume377Issue:4Pages:1284-1287
AbstractSmad7 is an antagonist of TGF-beta signaling pathway and the mechanism of its inhibitory effect is of great interest. We recently found that Smad7 could function in the nucleus by binding to the DNA elements containing the minimal Smad binding element CAGA box. In this work, we further applied single-molecule force spectroscopy to study the DNA-binding property of Smad7. Smad7 showed similar binding strength to the oligonucleotides corresponding to the CAGA-containing activin responsive element (ARE) and the PAI-1 promoter, as that of Smad4. However, Smad7 also exhibited a binding activity to the mutant ARE with the CAGA sequence substituted, indicating its DNA-binding specificity is different from other Smads. Moreover, we demonstrated that the MH2 domain of Smad7 had a higher binding affinity to the DNA elements than the full-length Smad7, while the N-terminal domain exhibited an inhibitory effect. (C) 2008 Elsevier Inc. All rights reserved.
KeywordTgf-beta Smad7 Smad4 Atomic Force Microscopy Single-molecule Force Spectroscopy Protein-dna Interaction
DOI10.1016/j.bbrc.2008.10.145
Indexed BySCI
Language英语
WOS IDWOS:000261630900050
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65294
Collection中国科学院化学研究所
Corresponding AuthorFang, Xiaohong
Affiliation1.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Tsinghua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
Shi, Xiaoli,Chen, Feng,Yu, Junping,et al. Study of interaction between Smad7 and DNA by single-molecule force spectroscopy[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,377(4):1284-1287.
APA Shi, Xiaoli.,Chen, Feng.,Yu, Junping.,Xu, Yongchun.,Zhang, Suping.,...&Fang, Xiaohong.(2008).Study of interaction between Smad7 and DNA by single-molecule force spectroscopy.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,377(4),1284-1287.
MLA Shi, Xiaoli,et al."Study of interaction between Smad7 and DNA by single-molecule force spectroscopy".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 377.4(2008):1284-1287.
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