ICCAS OpenIR
Detection of local polarity and conformational changes at the active site of rabbit muscle creatine kinase with a new arginine-specific fluorescent probe
Wang, Shujuan; Wang, Xiaochun; Shi, Wen; Wang, Ke; Ma, Huimin
2008-02-01
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
ISSN1570-9639
Volume1784Issue:2Pages:415-422
AbstractA new polarity-sensitive fluorescent probe, 3-(4-chloro-6-p-glyoxal-phenoxy- 1,3,5-triazinylamino)-7-(dimethylamino)-2-methylphenazine (CGTDP), is synthesized for selective labeling of active-site arginine residues. The probe comprises a neutral red moiety as a polarity-sensitive fluorophore and a phenylglyoxal unit as an arginine-specific labeling group. The probe exhibits a sensitive response of shift of fluorescence maximum emission wavelength to solvent polarity only instead of pH or temperature, which leads to the use of the probe in detecting the local polarity and conformational changes of the active site of rabbit muscle creatine kinase (CK) denatured by pH or temperature. The polarity of the active site domain has been first found to correspond to a dielectric constant of about 44, and the conformational change of the active site directly revealed by CGTDP occurs far before that of CK as a whole disclosed by the intrinsic tryptophan fluorescence during acid or thermal denaturation. The present strategy may provide a useful method to detect the local polarity and conformational changes of the active sites of many enzymes that employ arginine residues as anion recognition sites under different denaturation conditions. (c) 2007 Elsevier B.V All rights reserved.
KeywordArginine-specific Labeling Fluorescent Probes Active Site Local Polarity Detection Conformational Changes
DOI10.1016/j.bbapap.2007.11.009
Indexed BySCI
Language英语
WOS IDWOS:000253353600018
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65286
Collection中国科学院化学研究所
Corresponding AuthorMa, Huimin
AffiliationChinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wang, Shujuan,Wang, Xiaochun,Shi, Wen,et al. Detection of local polarity and conformational changes at the active site of rabbit muscle creatine kinase with a new arginine-specific fluorescent probe[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2008,1784(2):415-422.
APA Wang, Shujuan,Wang, Xiaochun,Shi, Wen,Wang, Ke,&Ma, Huimin.(2008).Detection of local polarity and conformational changes at the active site of rabbit muscle creatine kinase with a new arginine-specific fluorescent probe.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1784(2),415-422.
MLA Wang, Shujuan,et al."Detection of local polarity and conformational changes at the active site of rabbit muscle creatine kinase with a new arginine-specific fluorescent probe".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1784.2(2008):415-422.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Shujuan]'s Articles
[Wang, Xiaochun]'s Articles
[Shi, Wen]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Shujuan]'s Articles
[Wang, Xiaochun]'s Articles
[Shi, Wen]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Shujuan]'s Articles
[Wang, Xiaochun]'s Articles
[Shi, Wen]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.