ICCAS OpenIR
Aptamer-based affinity chromatographic assays for thrombin
Zhao, Qiang1; Li, Xing-Fang1; Shao, Yuanhua2; Le, X. Chris1,2
2008-10-01
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume80Issue:19Pages:7586-7593
AbstractAffinity chromatographic assays for thrombin were developed using two aptamers as affinity ligands. The efficient capture and step elution of thrombin with NaClO4 enabled the determination of thrombin by using either absorbance or fluorescence detection. Preconcentration of thrombin on the affinity column improved the detection limit of thrombin to 0.1 nM. Using an aptamer for the fibrinogenbinding site of thrombin and a second aptamer for the heparin-binding site, a sandwich chromatographic assay was developed, showing improved selectivity of thrombin detection and eliminating the need for labeling thrombin in the sample. The increased local concentration of aptamers immobilized on monolithic columns favored the formation of aptamer-thrombin complexes, resulting in improved retention and detection of thrombin at trace levels.
DOI10.1021/ac801206s
Indexed BySCI
Language英语
WOS IDWOS:000259603100057
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:84[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65108
Collection中国科学院化学研究所
Corresponding AuthorLe, X. Chris
Affiliation1.Univ Alberta, Dept Lab Med & Pathol, Div Analyt & Environm Toxicol, Edmonton, AB T6G 2G3, Canada
2.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Qiang,Li, Xing-Fang,Shao, Yuanhua,et al. Aptamer-based affinity chromatographic assays for thrombin[J]. ANALYTICAL CHEMISTRY,2008,80(19):7586-7593.
APA Zhao, Qiang,Li, Xing-Fang,Shao, Yuanhua,&Le, X. Chris.(2008).Aptamer-based affinity chromatographic assays for thrombin.ANALYTICAL CHEMISTRY,80(19),7586-7593.
MLA Zhao, Qiang,et al."Aptamer-based affinity chromatographic assays for thrombin".ANALYTICAL CHEMISTRY 80.19(2008):7586-7593.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao, Qiang]'s Articles
[Li, Xing-Fang]'s Articles
[Shao, Yuanhua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao, Qiang]'s Articles
[Li, Xing-Fang]'s Articles
[Shao, Yuanhua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao, Qiang]'s Articles
[Li, Xing-Fang]'s Articles
[Shao, Yuanhua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.