Aptamer-based affinity chromatographic assays for thrombin
Zhao, Qiang1; Li, Xing-Fang1; Shao, Yuanhua2; Le, X. Chris1,2
AbstractAffinity chromatographic assays for thrombin were developed using two aptamers as affinity ligands. The efficient capture and step elution of thrombin with NaClO4 enabled the determination of thrombin by using either absorbance or fluorescence detection. Preconcentration of thrombin on the affinity column improved the detection limit of thrombin to 0.1 nM. Using an aptamer for the fibrinogenbinding site of thrombin and a second aptamer for the heparin-binding site, a sandwich chromatographic assay was developed, showing improved selectivity of thrombin detection and eliminating the need for labeling thrombin in the sample. The increased local concentration of aptamers immobilized on monolithic columns favored the formation of aptamer-thrombin complexes, resulting in improved retention and detection of thrombin at trace levels.
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WOS IDWOS:000259603100057
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Cited Times:84[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorLe, X. Chris
Affiliation1.Univ Alberta, Dept Lab Med & Pathol, Div Analyt & Environm Toxicol, Edmonton, AB T6G 2G3, Canada
2.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Qiang,Li, Xing-Fang,Shao, Yuanhua,et al. Aptamer-based affinity chromatographic assays for thrombin[J]. ANALYTICAL CHEMISTRY,2008,80(19):7586-7593.
APA Zhao, Qiang,Li, Xing-Fang,Shao, Yuanhua,&Le, X. Chris.(2008).Aptamer-based affinity chromatographic assays for thrombin.ANALYTICAL CHEMISTRY,80(19),7586-7593.
MLA Zhao, Qiang,et al."Aptamer-based affinity chromatographic assays for thrombin".ANALYTICAL CHEMISTRY 80.19(2008):7586-7593.
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