ICCAS OpenIR
Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC
Yang Geng-Liang1,2; Bai Li-Gai1; Liu Hai-Yan1; Zhao Yu1; Li Feng-Xin1; Cao Wei-Min1
2008-11-01
Source PublicationCHINESE JOURNAL OF CHEMISTRY
ISSN1001-604X
Volume26Issue:11Pages:2063-2066
AbstractA novel cation-exchange polymeric short column based on poly(vinyl ester resin) monolith was applied to separation in our present work. The poly(vinyl ester resin) was used as the monomer as well as the crosslinking agent in the polymerization which was induced by the initiator 2-methylpropropionitrile (AIBN). 1-Hexadecanol Was used its the porogen and methanol was used to make the 1-hexadecanol resolve more easily. Morphology of the monolithic materials was Studied by scanning electron microscopy. The capabilities of separation and loading on this column in conjunction with HPLC were investigated, respectively. The effect of pH and concentration of mobile phase (buffer) on the elution of Lys was investigated. Moreover, Lys was separated from chicken egg white with high resolution in it short time in this experiment.
KeywordPoly(Vinyl Ester Resin) Polymeric Monoliths Hplc Separation
Indexed BySCI
Language英语
WOS IDWOS:000261672200019
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/65010
Collection中国科学院化学研究所
Corresponding AuthorYang Geng-Liang
Affiliation1.Hebei Univ, Coll Pharm, Baoding 071002, Hebei, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yang Geng-Liang,Bai Li-Gai,Liu Hai-Yan,et al. Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC[J]. CHINESE JOURNAL OF CHEMISTRY,2008,26(11):2063-2066.
APA Yang Geng-Liang,Bai Li-Gai,Liu Hai-Yan,Zhao Yu,Li Feng-Xin,&Cao Wei-Min.(2008).Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC.CHINESE JOURNAL OF CHEMISTRY,26(11),2063-2066.
MLA Yang Geng-Liang,et al."Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC".CHINESE JOURNAL OF CHEMISTRY 26.11(2008):2063-2066.
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