ICCAS OpenIR
Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation
Chen, Zhiyong1; Xu, Li2; Liang, Yuan1; Wang, Jianbin1; Zhao, Meiping1; Li, Yuanzong1
2008-02-22
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1182Issue:1Pages:128-131
AbstractA supermacroporous monolithic cryogel was directly prepared by in situ cryo-copolymerization in a stainless steel cartridge (70 mm x 5.0 mm I.D.) using methacrylic acid (MAA) as functional monomer;and polyethylene glycol diacrylate (PEGDA) as crosslinker. The highly crosslinked (90%, molar ratio) poly(MAA-PEGDA) cryogel had more uniform supermacropores with a mean diameter of 25 p,m compared to the poly(acrylamide)-based cryogels. The viability of poly(MAA-PEGDA) cryogel as a medium was demonstrated for separations of lysozyme from chicken egg white (CEW) and water-soluble poly(N-isopropylacrylamide-co-3-(dimethylamino) propyl methacrylamide) (NIPAM-DMAPMA) nanoparticles from its crude reaction solution. The dynamic binding capacities of lysozyme and the polymeric nanoparticles were 4.51 x 10(-3) mu mol/ml and 33.4 mu g/ml, respectively. The lysozyme recovered from the above separations had a purity of more than 85%, and retained 90% of its enzymatic activity. (c) 2008 Elsevier B.V. All rights reserved.
KeywordHplc Monolith Cryogel Nanoparticle Protein
DOI10.1016/j.chroma.2007.12.084
Indexed BySCI
Language英语
WOS IDWOS:000253869800013
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/64874
Collection中国科学院化学研究所
Corresponding AuthorZhao, Meiping
Affiliation1.Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, BNLMS, Beijing 100871, Peoples R China
2.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Chen, Zhiyong,Xu, Li,Liang, Yuan,et al. Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1182(1):128-131.
APA Chen, Zhiyong,Xu, Li,Liang, Yuan,Wang, Jianbin,Zhao, Meiping,&Li, Yuanzong.(2008).Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation.JOURNAL OF CHROMATOGRAPHY A,1182(1),128-131.
MLA Chen, Zhiyong,et al."Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation".JOURNAL OF CHROMATOGRAPHY A 1182.1(2008):128-131.
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