ICCAS OpenIR
The immobilization of basic fibroblast growth factor on plasma-treated poly(lactide-co-glycolide)
Shen, Hong; Hu, Xixue; Bei, Jianzhong; Wang, Shenguo
2008-05-01
Source PublicationBIOMATERIALS
ISSN0142-9612
Volume29Issue:15Pages:2388-2399
AbstractIn this study, possibility of the method of immobilization of basic fibroblast growth factor (bFGF) on polylactone-type polymer scaffolds via plasma treatment was investigated. To introduce acid carboxylic functional groups on the surface of the polymer matrix, poly(lactide-co-glycolide) (PLGA) film was treated with carbon dioxide (CO2) plasma and then incubated in a phosphate buffer saline (PBS, pH 7.4) solution of bFGF. The bFGF binding efficiency to the CO2 plasma-treated PLGA (PT-PLGA) films under different treating parameters was investigated and compared. It was found bFGF binding efficiency to PLGA was enhanced by CO2 plasma treatment. The binding efficiency of bFGF to PLGA was variational with CO2 plasma treating time and it reached a maximum after a treating time of 20 min under the power of 20 W. The changes of surface chemistry and surface topography induced by CO2 plasma treatment played main roles in improving binding efficiency. Bound bFGF was released continuously from the films for up to 7 days in vitro. The stability of bFGF immobilized on PLGA film via CO2 plasma treatment was tested further under dynamic conditions by a Parallel Plate How Chamber. Mouse 3T3 fibroblasts were cultured on the bFGF bound PLGA with a prior plasma treatment (20 W, 20 min) (PT-PLGA/bFGF) film, which showed that bFGF released from PT-PLGA/bFGF film was bioactive. Adhesion and growth of cells on PLGA scaffolds were greatly improved by immobilization of bFGF on them. Therefore, the method Of CO2 plasma treatment combining bFGF anchorage not only was usable in delivering bFGF, but also could be applied extensively for surface modification of scaffolds in tissue engineering. (c) 2008 Elsevier Ltd. All rights reserved.
KeywordBasic Fibroblast Growth Factor (Bfgf) Deliver Vehicle Plga Scaffold Co2 Plasma Treatment Surface Modification Tissue Engineering
DOI10.1016/j.biomaterials.2008.02.008
Indexed BySCI
Language英语
WOS IDWOS:000255088700010
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:82[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/64502
Collection中国科学院化学研究所
Corresponding AuthorWang, Shenguo
AffiliationChinese Acad Sci, Inst Chem, BNLMS, State Key Lab Polymer Phys & Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Shen, Hong,Hu, Xixue,Bei, Jianzhong,et al. The immobilization of basic fibroblast growth factor on plasma-treated poly(lactide-co-glycolide)[J]. BIOMATERIALS,2008,29(15):2388-2399.
APA Shen, Hong,Hu, Xixue,Bei, Jianzhong,&Wang, Shenguo.(2008).The immobilization of basic fibroblast growth factor on plasma-treated poly(lactide-co-glycolide).BIOMATERIALS,29(15),2388-2399.
MLA Shen, Hong,et al."The immobilization of basic fibroblast growth factor on plasma-treated poly(lactide-co-glycolide)".BIOMATERIALS 29.15(2008):2388-2399.
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