ICCAS OpenIR
Fluorescence turn-on detection of DNA and label-free fluorescence nuclease assay based on the aggregation-induced emission of silole
Wang, Ming1,2; Zhang, Deqing1; Zhang, Guanxin1; Tang, Yanli1,2; Wang, Shu1; Zhu, Daoben1
2008-08-15
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume80Issue:16Pages:6443-6448
AbstractBy making use of the aggregation-induced emission feature of silole, compound 1 with an ammonium group is designed and synthesized with a view to developing a new optical probe for fluorescence turn-on detection of DNA and label-free fluorescence nuclease assay. The fluorescence of 1 increases largely upon mixing with DNA, in particular for long DNA, indicating that I can be used for fluorescence turn-on detection of DNA. More interestingly, 1 can be employed to follow the DNA cleavage process by nuclease. Therefore, a label-free fluorescence nuclease assay method is successfully established with 1. Furthermore, this label-free fluorescence assay can also be used for inhibitor screening of nucleases. Given its simplicity, easy operation, sensitivity and cost-effectiveness, this method can be extended to other nuclease assays and high-throughput screening of nuclease inhibitors.
DOI10.1021/ac801020v
Indexed BySCI
Language英语
WOS IDWOS:000258448100040
PublisherAMER CHEMICAL SOC
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/64440
Collection中国科学院化学研究所
Corresponding AuthorZhang, Deqing
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Organ Solids Lab, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ming,Zhang, Deqing,Zhang, Guanxin,et al. Fluorescence turn-on detection of DNA and label-free fluorescence nuclease assay based on the aggregation-induced emission of silole[J]. ANALYTICAL CHEMISTRY,2008,80(16):6443-6448.
APA Wang, Ming,Zhang, Deqing,Zhang, Guanxin,Tang, Yanli,Wang, Shu,&Zhu, Daoben.(2008).Fluorescence turn-on detection of DNA and label-free fluorescence nuclease assay based on the aggregation-induced emission of silole.ANALYTICAL CHEMISTRY,80(16),6443-6448.
MLA Wang, Ming,et al."Fluorescence turn-on detection of DNA and label-free fluorescence nuclease assay based on the aggregation-induced emission of silole".ANALYTICAL CHEMISTRY 80.16(2008):6443-6448.
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