Glycoproteins analysis by surface plasmon resonance absorption and imaging
Liu Wei1,2; Chen Yi1
AbstractSurface plasmon resonance(SPR) absorption and imaging(SPRI) have been explored for screening and differentiating glycoproteins from non-glycoprotein according to the shift of maximum SPR absorption and/or to the variation of picture gray intensity. In combination with competitive washing with sugar solutions, SPRI has the ability to produce different signals for different gylcoproteins, offering a potential way to identify these gylcoconjugates. To conduct SPR and SPRI, proteins(at 1.0 mg/mL), in stead of lectins, were immobilized on gold sensing films to reduce the use of the costly glycoproteins. The sensing film was fixed in a laboratory-established SPR or SPRI system, equilibrated with 50 mmol/L Tris-HCl at pH = 7. 4 at a flow rate of 20 mu L/min, reacted with 200 nmol/L ConA(prepared in the Tris-HCl solution containing 120 mmol/L NaCl, 25 mmol/L CaCl2 and 5 mmol/L MnCl2) at room temperature for 1 h and rewashed with Tris-HCl solution. For competitive washing, 80 mmol/L glucose solution was pumped into the analytical cell and reacted for 1 h. The signals were recorded whenever necessary. Non-gylcoproteins had no signals when using either SPR or SPRI while glycoproteins gave different SPR shifts and gray intensity in SPRI.
KeywordGlycoprotein Protein Microarray Surface Plasmon Resonance Imaging(Spri)
Indexed BySCI
WOS IDWOS:000259522800008
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorChen Yi
Affiliation1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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GB/T 7714
Liu Wei,Chen Yi. Glycoproteins analysis by surface plasmon resonance absorption and imaging[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2008,29(9):1744-1746.
APA Liu Wei,&Chen Yi.(2008).Glycoproteins analysis by surface plasmon resonance absorption and imaging.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,29(9),1744-1746.
MLA Liu Wei,et al."Glycoproteins analysis by surface plasmon resonance absorption and imaging".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 29.9(2008):1744-1746.
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