ICCAS OpenIR
Unlabeled hairpin-DNA probe for the detection of single-nucleotide mismatches by electrochemical impedance spectroscopy
Wang, Ying1; Li, Congjuan1; Li, Xiaohong1; Li, Yongfang3; Kraatz, Heinz-Bernhard2
2008-03-15
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume80Issue:6Pages:2255-2260
AbstractAn unlabeled hairpin-DNA probe was used for the detection of eight single-nucleotide mismatches by electrochemical impedance spectroscopy (EIS). Upon hybridization of the target strand with the hairpin DNA probe, the stem-loop structure is opened and forms a duplex DNA. Accordingly, the film thickness is increased, which causes differences in the electrical properties of the film before and after hybridization. Randles equivalent circuits were employed to evaluate the EIS result. The differences in the charge-transfer resistance Delta R-CT between hairpin DNA (before hybridization) and duplex DNA (after hybridization) shows the consequence of a large structural rearrangement from hairpin to duplex. If a single-nucleotide mismatch is present in the center of the duplex, the difference in charge-transfer resistance Delta R-CT between B-DNA. in the absence and presence of Zn2+ allows the unequivocal detection of all eight single-nucleotide mismatches. The detection limit was measured, and Delta R-CT allows the discrimination of a single-nucleotide mismatch with the concentration of the target strand as low as 10 pM.
DOI10.1021/ac7024688
Indexed BySCI
Language英语
WOS IDWOS:000254017000055
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:59[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/63984
Collection中国科学院化学研究所
Corresponding AuthorLi, Xiaohong
Affiliation1.Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China
2.Univ Western Ontario, Dept Chem, London, ON N6A 5B7, Canada
3.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Key Lab Organ Solids, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ying,Li, Congjuan,Li, Xiaohong,et al. Unlabeled hairpin-DNA probe for the detection of single-nucleotide mismatches by electrochemical impedance spectroscopy[J]. ANALYTICAL CHEMISTRY,2008,80(6):2255-2260.
APA Wang, Ying,Li, Congjuan,Li, Xiaohong,Li, Yongfang,&Kraatz, Heinz-Bernhard.(2008).Unlabeled hairpin-DNA probe for the detection of single-nucleotide mismatches by electrochemical impedance spectroscopy.ANALYTICAL CHEMISTRY,80(6),2255-2260.
MLA Wang, Ying,et al."Unlabeled hairpin-DNA probe for the detection of single-nucleotide mismatches by electrochemical impedance spectroscopy".ANALYTICAL CHEMISTRY 80.6(2008):2255-2260.
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