ICCAS OpenIR
Aptamer-based electrochemical sensors with aptamer-complementary DNA oligonucleotides as probe
Lu, Ying1,2; Li, Xianchan1,2; Zhang, Limin1,2; Yu, Ping1; Su, Lei1; Mao, Lanqun1
2008-03-15
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume80Issue:6Pages:1883-1890
AbstractThis study describes a facile and general strategy for the development of aptamer-based electrochemical sensors with a high specificity toward the targets and a ready regeneration feature. Very different from the existing strategies for the development of electrochemical aptasensors with the aptamers as the probes, the strategy proposed here is essentially based on the utilization of the aptamer-complementary DNA (cDNA) oligonucleotides as the probes for electrochemical sensing. In this context, the sequences at both ends of the cDNA are tailor-made to be complementary and both the redox moiety (i.e., ferrocene in this study) and thiol group are labeled onto the cDNA. The labeled cDNA are hybridized with their respective aptamers (i.e., ATP- and thrombin-binding aptamers in this study) to form double-stranded DNA (dsDNA) and the electrochemical aptasensors are prepared by self-assembling the labeled ds-DNA onto Au electrodes. Upon target binding, the aptamers confined onto electrode surface dissociate from their respective cDNA oligonucleotides into the solution and the single-stranded cDNA could thus tend to form a hairpin structure through the hybridization of the complementary sequences at both its ends. Such a conformational. change of the cDNA resulting from the target binding-induced dissociation of the aptamers essentially leads to the change in the voltammetric signal of the redox moiety labeled onto the cDNA and thus constitutes the mechanism for the electrochemical aptasensors for specific target sensing. The aptasensors demonstrated here with the cDNA as the probe are readily regenerated and show good responses toward the targets. This study may offer a new and relatively general approach to electrochemical aptasensors with good analytical properties and potential applications.
DOI10.1021/ac7018014
Indexed BySCI
Language英语
WOS IDWOS:000254017000007
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:163[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/63974
Collection中国科学院化学研究所
Corresponding AuthorMao, Lanqun
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Lu, Ying,Li, Xianchan,Zhang, Limin,et al. Aptamer-based electrochemical sensors with aptamer-complementary DNA oligonucleotides as probe[J]. ANALYTICAL CHEMISTRY,2008,80(6):1883-1890.
APA Lu, Ying,Li, Xianchan,Zhang, Limin,Yu, Ping,Su, Lei,&Mao, Lanqun.(2008).Aptamer-based electrochemical sensors with aptamer-complementary DNA oligonucleotides as probe.ANALYTICAL CHEMISTRY,80(6),1883-1890.
MLA Lu, Ying,et al."Aptamer-based electrochemical sensors with aptamer-complementary DNA oligonucleotides as probe".ANALYTICAL CHEMISTRY 80.6(2008):1883-1890.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lu, Ying]'s Articles
[Li, Xianchan]'s Articles
[Zhang, Limin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lu, Ying]'s Articles
[Li, Xianchan]'s Articles
[Zhang, Limin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lu, Ying]'s Articles
[Li, Xianchan]'s Articles
[Zhang, Limin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.