ICCAS OpenIR
Conjugation of a water-soluble gadolinium endohedral fulleride with an antibody as a magnetic resonance imaging contrast agent
Shu, Chun-Ying1; Ma, Xin-Yong1; Zhang, Jian-Fei2; Corwin, Frank D.3; Sim, Jae Hyun2; Zhang, Er-Yun1; Dorn, Harry C.2; Gibson, Harry W.2; Fatouros, Panos P.3; Wang, Chun-Ru1; Fang, Xiao-Hong1
2008-03-01
Source PublicationBIOCONJUGATE CHEMISTRY
ISSN1043-1802
Volume19Issue:3Pages:651-655
AbstractWater-soluble gadofullerides exhibited high efficiency as magnetic resonance imaging (MRI) contrast agents. In this paper, we report the conjugation of the newly synthesized gadofulleride, Gd@C82O6(OH)(16)(-)(NHCH2CH2COOH)(8), with the antibody of green fluorescence protein (anti-GFP), as a model for "tumor targeted" imaging agents based on endohedral metallofullerenes. In this model system, the activity of the anti-GFP conjugate can be conveniently detected by green fluorescence protein (GFP), leading to in vitro experiments more direct and facile than those of tumor antibodies. Objective-type total internal reflection fluorescence microscopy revealed that each gadofulleride aggregate conjugated on average five anti-GFPs, and the activity of anti-GFPs was preserved after conjugation. In addition, the gadofulleride/antibody conjugate exhibited higher water proton relaxivity (12.0 mM(-1) s(-1)) than the parent gadofulleride aggregate (8.1 mM(-1) s(-1)) in phosphate buffered saline at 0.35 T, as also confirmed by T-1-weighted images of phantoms. These observations clearly indicate that the synthesized gadofulleride/antibody conjugate not only has targeting potential, but also exhibits higher efficiency as an MRI contrast agent.
DOI10.1021/bc7002742
Indexed BySCI
Language英语
WOS IDWOS:000254185000011
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/63732
Collection中国科学院化学研究所
Corresponding AuthorShu, Chun-Ying
Affiliation1.Chinese Acad Sci, BNLMS, Inst Chem, Beijing 100080, Peoples R China
2.Virginia Polytech Inst & State Univ, Dept Chem, Blacksburg, VA 24060 USA
3.Virginia Commonwealth Univ, Dept Radiol, Richmond, VA 23298 USA
Recommended Citation
GB/T 7714
Shu, Chun-Ying,Ma, Xin-Yong,Zhang, Jian-Fei,et al. Conjugation of a water-soluble gadolinium endohedral fulleride with an antibody as a magnetic resonance imaging contrast agent[J]. BIOCONJUGATE CHEMISTRY,2008,19(3):651-655.
APA Shu, Chun-Ying.,Ma, Xin-Yong.,Zhang, Jian-Fei.,Corwin, Frank D..,Sim, Jae Hyun.,...&Fang, Xiao-Hong.(2008).Conjugation of a water-soluble gadolinium endohedral fulleride with an antibody as a magnetic resonance imaging contrast agent.BIOCONJUGATE CHEMISTRY,19(3),651-655.
MLA Shu, Chun-Ying,et al."Conjugation of a water-soluble gadolinium endohedral fulleride with an antibody as a magnetic resonance imaging contrast agent".BIOCONJUGATE CHEMISTRY 19.3(2008):651-655.
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