ICCAS OpenIR
Development and characterization of an immunoaffinity monolith for selective on-line extraction of bisphenol A from environmental water samples
Li, Lin; Wang, Jianbin; Zhou, Shuang; Zhao, Meiping
2008-07-14
Source PublicationANALYTICA CHIMICA ACTA
ISSN0003-2670
Volume620Issue:1-2Pages:1-7
AbstractMatrix interference and contamination from analytical procedures are two major issues in the detection of trace level of bisphenol A (BPA) in environmental water. In this paper, we report a highly selective and efficient method for on-line extraction of BPA from water samples followed by quantification with liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Poly(ethylene dimethacrylate-glycidyl methacrylate) monolith was synthesized in 50 mm x 4.6 mm i.d. stainless steel cartridges and the epoxy-groups on the surface of the monolith were hydrolyzed and oxidized to aldehyde functions. Antibodies against BPA were covalently immobilized onto the monolithic column via Schiff base reaction. The optimum application buffer and elution buffer were found to be pH 5.5 phosphate buffered saline (PBS) and methanol-water (70:30, v/v), respectively. The obtained immunoaffinity monolithic columns were on-line coupled to LC-MS/MS using column-switching valves and the system was applied to analyze BPA in real environmental water samples. The method achieved a detection limit of 0.3 ng L-1 using a sample volume of 100 mL. The linear calibration range was 1.0-160 ng L-1. Samples including tap water, lake water and effluent from municipal sewage treatment plant were all measured with satisfactory results. (c) 2008 Elsevier B.V. All rights reserved.
KeywordImmunoaffinity Monolith Bisphenol a Liquid Chromatography
DOI10.1016/j.aca.2008.05.036
Indexed BySCI
Language英语
WOS IDWOS:000257553200001
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/63602
Collection中国科学院化学研究所
Corresponding AuthorZhao, Meiping
AffiliationPeking Univ, BNLMS, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Li, Lin,Wang, Jianbin,Zhou, Shuang,et al. Development and characterization of an immunoaffinity monolith for selective on-line extraction of bisphenol A from environmental water samples[J]. ANALYTICA CHIMICA ACTA,2008,620(1-2):1-7.
APA Li, Lin,Wang, Jianbin,Zhou, Shuang,&Zhao, Meiping.(2008).Development and characterization of an immunoaffinity monolith for selective on-line extraction of bisphenol A from environmental water samples.ANALYTICA CHIMICA ACTA,620(1-2),1-7.
MLA Li, Lin,et al."Development and characterization of an immunoaffinity monolith for selective on-line extraction of bisphenol A from environmental water samples".ANALYTICA CHIMICA ACTA 620.1-2(2008):1-7.
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