ICCAS OpenIR
Synergistic Design of Electric Field and Membrane in Facilitating Continuous Adsorption for Cleanup and Enrichment of Proteins in Direct ESI-MS Analysis
Zhou, Yu1; Shen, Hailin2; Yi, Tie3; Wen, Dawei1; Pang, Nannan1; Liao, Jie4; Liu, Huwei1
2008-12-01
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume80Issue:23Pages:8920-8929
AbstractWe designed and fabricated a novel microdevice to facilitate continuous adsorption phenomena for biological sample preparation. Using the device, we also developed an online, highly integrated, multifunctional strategy, with a promise of accepting a large volume of crude tissue extracts with the end point generation of a reliable MS identification within 20 min. Under an external electric field, charged membranes can adsorb multiple layers of proteins, which exceed the capacity limit of common resins or membranes. It enlarges sample loading and trapping efficiency, thus bypasses the tradeoff between sample capacity and downstream detection sensitivity. This integrated approach, formed by synergistic utilization among electric field, membrane, and fluidic handling at the microscale, reduces the overall complexity of crude samples in one step for direct MS analysis. The sample preparation goals, including enrichment, desalting, removal of noncharged contaminants, and initial fractionation, can be rapidly performed in a single device. The strategy facilitates reproducible MS quantification by circumventing traditional laborious and time-consuming sample preparation steps. In addition, MEPD extended the ion trap linear dynamic range from 2 to at least 4 orders of magnitude by eliminating ion suppression effect, enriching target analyte(s), and decreasing sample loss during integrated sample preparation.
DOI10.1021/ac800816k
Indexed BySCI
Language英语
WOS IDWOS:000261335600012
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/63568
Collection中国科学院化学研究所
Corresponding AuthorLiu, Huwei
Affiliation1.Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem,Beijing Natl Lab Mol Sci, Minist Educ,Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China
2.Univ Manchester, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England
3.Cent S Univ, Coll Informat Sci & Engn, Changsha 410083, Peoples R China
4.Gen Hosp Chinese PLA, Med Expt & Anal Ctr, Beijing 100853, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Yu,Shen, Hailin,Yi, Tie,et al. Synergistic Design of Electric Field and Membrane in Facilitating Continuous Adsorption for Cleanup and Enrichment of Proteins in Direct ESI-MS Analysis[J]. ANALYTICAL CHEMISTRY,2008,80(23):8920-8929.
APA Zhou, Yu.,Shen, Hailin.,Yi, Tie.,Wen, Dawei.,Pang, Nannan.,...&Liu, Huwei.(2008).Synergistic Design of Electric Field and Membrane in Facilitating Continuous Adsorption for Cleanup and Enrichment of Proteins in Direct ESI-MS Analysis.ANALYTICAL CHEMISTRY,80(23),8920-8929.
MLA Zhou, Yu,et al."Synergistic Design of Electric Field and Membrane in Facilitating Continuous Adsorption for Cleanup and Enrichment of Proteins in Direct ESI-MS Analysis".ANALYTICAL CHEMISTRY 80.23(2008):8920-8929.
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