ICCAS OpenIR
Efficient expression in E-coli of an enantioselective nitrile hydratase from Rhodococcus erythropolis
Song, Liya1,2,3; Yuan, Hong-Jie1,4; Coffey, Lee1; Doran, John1; Wang, Mei-Xiang4; Qian, Shijun2; O'Reilly, Catherine1
2008-04-01
Source PublicationBIOTECHNOLOGY LETTERS
ISSN0141-5492
Volume30Issue:4Pages:755-762
AbstractThe genes encoding an enantioselective nitrile hydratase (NHase) from Rhodococcus erythropolis AJ270 have been cloned and an active NHase has been produced in Escherichia coli. Maximal activity was found when the genes encoding the alpha- and beta-subunits were transcribed as one unit and the gene encoding the P44k activator protein as a separate ORF on a single replicon. Addition of n-butyric acid and FeSO4 could improve NHase activity. Coexpression of the GroEL-GroES chaperone proteins increased activity in the absence of P44k protein but had no effect in the presence of P44k. The recombinant enzyme was highly enantioselective in the synthesis of S-(+)-3-benzoyloxy- 4-cyanobutyramide from the prochiral substrate 3-benzoyloxyglutaronitrile.
KeywordActivator Protein Enantioselectivity Groes-groel Nitrile Hydratase Rhodococcus Erythropolis
DOI10.1007/s10529-007-9611-3
Indexed BySCI
Language英语
WOS IDWOS:000253357800028
PublisherSPRINGER
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/63376
Collection中国科学院化学研究所
Corresponding AuthorO'Reilly, Catherine
Affiliation1.Waterford Inst Technol, Dept Chem & Life Sci, Waterford, Ireland
2.Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Biol Chem Lab, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Song, Liya,Yuan, Hong-Jie,Coffey, Lee,et al. Efficient expression in E-coli of an enantioselective nitrile hydratase from Rhodococcus erythropolis[J]. BIOTECHNOLOGY LETTERS,2008,30(4):755-762.
APA Song, Liya.,Yuan, Hong-Jie.,Coffey, Lee.,Doran, John.,Wang, Mei-Xiang.,...&O'Reilly, Catherine.(2008).Efficient expression in E-coli of an enantioselective nitrile hydratase from Rhodococcus erythropolis.BIOTECHNOLOGY LETTERS,30(4),755-762.
MLA Song, Liya,et al."Efficient expression in E-coli of an enantioselective nitrile hydratase from Rhodococcus erythropolis".BIOTECHNOLOGY LETTERS 30.4(2008):755-762.
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