ICCAS OpenIR
Comparative study of change in extracellular ascorbic acid in different brain ischemia/reperfusion models with in vivo microdialysis combined with on-line electrochemical detection
Liu, Kun1,2; Lin, Yuqing1,2; Xiang, Ling1; Yu, Ping1; Su, Lei1; Mao, Lanqun1
2008-05-01
Source PublicationNEUROCHEMISTRY INTERNATIONAL
ISSN0197-0186
Volume52Issue:6Pages:1247-1255
AbstractInformation on the change in extracellular ascorbic acid (AA) during the acute period of cerebral ischemia is of great importance in the early therapeutic intervention of the cerebral ischemic injury since AA is known to be involved into most kinds of neurochemical changes in the cerebral ischemia. This study describes a fast and efficient method through integration of in vivo microdialysis with on-line electrochemical detection for continuous monitoring cerebral AA, allowing comparative study of the change in the extracellular AA level in different brain ischemia/reperfusion models. The method exhibits a high specificity for AA measurements, bearing a good tolerance against the fluctuation in the brain anoxia and acidity induced by cerebral ischemia/reperfusion. In the global two-vessel occlusion (2-VO) ischemia model, the striatum AA did not change with statistic significance until 60 min after occlusion and was decreased to be 91 +/- 3% (n = 5, P < 0.05) of the basal level (8.05 +/- 0.23 mu M) at the time point of 60 min after occlusion. In the 2-VO ischemia/reperfusion model, AA remained unchanged during the 10 min of ischemia, and was sharply increased to be 267 +/- 74% (n = 5, P < 0.05) of the basal level after the initial 15 min of reperfusion, and then decreased to be 122 +/- 33% (n = 5, P < 0.05) of the basal level after 50 min of reperfusion. Extracellular AA was largely increased after 5 min of left middle cerebral artery occlusion (LMCAO) and was then gradually increased to be 257 +/- 49% (n = 5, P < 0.05) of the basal level after 60 min of LMCAO ischemia. In the LMCAO ischemia/reperfusion model, AA was greatly increased during 10 min of ischemia and then gradually increased to be 309 +/- 69% (it = 5, P < 0.05) of the basal level after the consecutive 50 min of reperfusion. The results demonstrated here may be useful for understanding the neurochemical processes in the acute period of cerebral ischemia and could thus be important for neuroprotective therapeutics for cerebral ischemic injury. (C) 2008 Elsevier Ltd. All rights reserved.
KeywordIn Vivo Microdialysis On-line Electrochemical Detection Ascorbic Acid Cerebral Ischemia/reperfusion Striatum
DOI10.1016/j.neuint.2008.01.006
Indexed BySCI
Language英语
WOS IDWOS:000255431200039
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:43[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/63290
Collection中国科学院化学研究所
Corresponding AuthorMao, Lanqun
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
2.CAS, Grad Sch, Beijing 100086, Peoples R China
Recommended Citation
GB/T 7714
Liu, Kun,Lin, Yuqing,Xiang, Ling,et al. Comparative study of change in extracellular ascorbic acid in different brain ischemia/reperfusion models with in vivo microdialysis combined with on-line electrochemical detection[J]. NEUROCHEMISTRY INTERNATIONAL,2008,52(6):1247-1255.
APA Liu, Kun,Lin, Yuqing,Xiang, Ling,Yu, Ping,Su, Lei,&Mao, Lanqun.(2008).Comparative study of change in extracellular ascorbic acid in different brain ischemia/reperfusion models with in vivo microdialysis combined with on-line electrochemical detection.NEUROCHEMISTRY INTERNATIONAL,52(6),1247-1255.
MLA Liu, Kun,et al."Comparative study of change in extracellular ascorbic acid in different brain ischemia/reperfusion models with in vivo microdialysis combined with on-line electrochemical detection".NEUROCHEMISTRY INTERNATIONAL 52.6(2008):1247-1255.
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