ICCAS OpenIR
Excitation dynamics of two spectral forms of the core complexes from photosynthetic bacterium Thermochromatium tepidum
Ma, Fei1,2; Kimura, Yukihiro3; Zhao, Xiao-Hui1,2; Wu, Yi-Shi1,2; Wang, Peng2; Fu, Li-Min2; Wang, Zheng-Yu3; Zhang, Jian-Ping1,2
2008-10-01
Source PublicationBIOPHYSICAL JOURNAL
ISSN0006-3495
Volume95Issue:7Pages:3349-3357
AbstractThe intact core antenna-reaction center (LH1-RC) core complex of thermophilic photosynthetic bacterium Thermochromatium(Tch.) tepidum is peculiar in its long-wavelength LH1-Q(y) absorption (915 nm). We have attempted comparative studies on the excitation dynamics of bacteriochlorophyll (BChl) and carotenoid (Car) between the intact core complex and the EDTA-treated one with the Q(y) absorption at 889 nm. For both spectral forms, the overall Car-to-BChl excitation energy transfer efficiency is determined to be similar to 20%, which is considerably lower than the reported values, e. g.,similar to 35%, for other photosynthetic purple bacteria containing the samekind of Car (spirilloxanthin). The RC trapping time constants are found to be 50 similar to 60 ps (170 similar to 200 ps) forRCin open (closed) state irrespective to the spectral forms and the wavelengths of Q(y) excitation. Despitethe low-energy LH1-Q(y) absorption, the RC trapping time are comparable to those reported for other photosynthetic bacteria with normal LH1-Q(y) absorption at 880 nm. Selective excitation to Car results in distinct differences in the Q(y)- bleaching dynamics between the two different spectral forms. This, together with the Car band-shift signals in response to Q(y) excitation, reveals the presence of two major groups of BChls in the LH1 of Tch. tepidum with a spectral heterogeneity of similar to 240 cm(-1), as well as an alteration in BChl-Car geometry in the 889-nm preparation with respect to the native one.
DOI10.1529/biophysj.108.133835
Indexed BySCI
Language英语
WOS IDWOS:000259393200027
PublisherBIOPHYSICAL SOC
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/63228
Collection中国科学院化学研究所
Corresponding AuthorZhang, Jian-Ping
Affiliation1.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
2.Renmin Univ China, Dept Chem, Beijing, Peoples R China
3.Ibaraki Univ, Fac Sci, Mito, Ibaraki 310, Japan
Recommended Citation
GB/T 7714
Ma, Fei,Kimura, Yukihiro,Zhao, Xiao-Hui,et al. Excitation dynamics of two spectral forms of the core complexes from photosynthetic bacterium Thermochromatium tepidum[J]. BIOPHYSICAL JOURNAL,2008,95(7):3349-3357.
APA Ma, Fei.,Kimura, Yukihiro.,Zhao, Xiao-Hui.,Wu, Yi-Shi.,Wang, Peng.,...&Zhang, Jian-Ping.(2008).Excitation dynamics of two spectral forms of the core complexes from photosynthetic bacterium Thermochromatium tepidum.BIOPHYSICAL JOURNAL,95(7),3349-3357.
MLA Ma, Fei,et al."Excitation dynamics of two spectral forms of the core complexes from photosynthetic bacterium Thermochromatium tepidum".BIOPHYSICAL JOURNAL 95.7(2008):3349-3357.
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