ICCAS OpenIR
Noninvasive optical Imaging of Staphylococcus aureus bacterial infection in living mice using a bis-dipicolylamine-zinc(II) affinity group conjugated to a near-infrared fluorophore
Leevy, W. Matthew1,2; Gammon, Seth T.3; Johnson, James R.1,2; Lampkins, Andrew J.1,2; Jiang, Hua1,2,4; Marquez, Manuel5,6; Piwnica-Worms, David3; Suckow, Mark A.7; Smith, Bradley D.1,2
2008-03-01
Source PublicationBIOCONJUGATE CHEMISTRY
ISSN1043-1802
Volume19Issue:3Pages:686-692
AbstractOptical imaging of bacterial infection in living animals is usually conducted with genetic reporters such as light-emitting enzymes or fluorescent proteins. However, there are many circumstances where genetic reporters are not applicable, and there is a need for exogenous synthetic probes that can selectively target bacteria. The focus of this study is a fluorescent imaging probe that is composed of a bacterial affinity group conjugated to a near-infrared dye. The affinity group is a synthetic zinc (II) coordination complex that targets the anionic surfaces of bacterial cells. The probe allows detection of Staphylococcus aureus infection (5 x 10(7) cells) in a mouse leg infection model using whole animal near-infrared fluorescence imaging. Region of interest analysis showed that the signal ratio for infected leg to uninfected leg reaches 3.9 +/- 0.5 at 21 h postinjection of the probe. Ex vivo imaging of the organs produced a signal ratio of 8 for infected to uninfected leg. Immunohistochemical analysis confirmed that the probe targeted the bacterial cells in the infected tissue. Optimization of the imaging filter set lowered the background signal due to autofluorescence and substantially improved imaging contrast. The study shows that near-infrared molecular probes are amenable to noninvasive optical imaging of localized S. aureus infection.
DOI10.1021/bc700376v
Indexed BySCI
Language英语
WOS IDWOS:000254185000016
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:83[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/63102
Collection中国科学院化学研究所
Corresponding AuthorLeevy, W. Matthew
Affiliation1.Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
2.Univ Notre Dame, Walther Canc Res Ctr, Notre Dame, IN 46556 USA
3.Washington Univ, Sch Med, Mallinckrodt Inst Radiol, Mol Imaging Ctr, St Louis, MO 63110 USA
4.Chinese Acad Sci, Inst Chem, Key Lab Photochem, Beijing 100080, Peoples R China
5.NIST, Ctr Theoret & Computat Nanosci, Gaithersburg, MD 20899 USA
6.Arizona State Univ, Harrington Dept Bioengn, Tempe, AZ 85287 USA
7.Univ Notre Dame, Freimann Life Sci Ctr, Notre Dame, IN 46556 USA
Recommended Citation
GB/T 7714
Leevy, W. Matthew,Gammon, Seth T.,Johnson, James R.,et al. Noninvasive optical Imaging of Staphylococcus aureus bacterial infection in living mice using a bis-dipicolylamine-zinc(II) affinity group conjugated to a near-infrared fluorophore[J]. BIOCONJUGATE CHEMISTRY,2008,19(3):686-692.
APA Leevy, W. Matthew.,Gammon, Seth T..,Johnson, James R..,Lampkins, Andrew J..,Jiang, Hua.,...&Smith, Bradley D..(2008).Noninvasive optical Imaging of Staphylococcus aureus bacterial infection in living mice using a bis-dipicolylamine-zinc(II) affinity group conjugated to a near-infrared fluorophore.BIOCONJUGATE CHEMISTRY,19(3),686-692.
MLA Leevy, W. Matthew,et al."Noninvasive optical Imaging of Staphylococcus aureus bacterial infection in living mice using a bis-dipicolylamine-zinc(II) affinity group conjugated to a near-infrared fluorophore".BIOCONJUGATE CHEMISTRY 19.3(2008):686-692.
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