ICCAS OpenIR
Molecular interaction and energy transfer between human serum albumin and bioactive component Aloe dihydrocoumarin
Zhang, Xiu-Feng; Xie, Ling; Liu, Yang; Xiang, Jun-Feng; Li, Lin; Tang, Ya-Lin
2008-10-15
Source PublicationJOURNAL OF MOLECULAR STRUCTURE
ISSN0022-2860
Volume888Issue:1-3Pages:145-151
AbstractAloe dihydrocoumarin is an antioxidant and a candidate of immunomodulatory drug on the immune system and can balance physiological reactive oxygen species (ROS) levels which may be useful to maintain homeostasis. In order to explore the mechanism of drug action at a molecular level, the binding of Aloe dihydrocoumarin with human serum albumin (HSA) has been investigated by fluorescence, ultraviolet (UV), circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy, fluorescence dynamics, and molecular dynamic docking for the first time. We observed a quenching of fluorescence of HSA in the presence of Aloe dihydrocoumarin and also analyzed the quenching results using the Stern-Volmer equation and obtained high affinity binding to HSA. A Forster type fluorescence resonance energy transfer mechanism is involved in this quenching of Trp fluorescence by Aloe dihydrocoumarin. From the CD and FT-IR results, it is apparent that the interaction of Aloe dihydrocoumarin with HSA causes a conformational change of the protein, with the loss of a-helix stability and the gain of beta-sheet and beta-turn content. Data obtained by fluorescence spectroscopy, fluorescence dynamics, CD, and FT-IR experiments along with the docking studies suggest that Aloe dihydrocoumarin binds to residues located in subdomain IIA of HSA. (C) 2007 Elsevier B.V. All rights reserved.
KeywordHuman Serum Albumin Aloe Dihydrocoumarin Fluorescent Quenching Fluorescence Dynamics Cd Ft-ir Docking
DOI10.1016/j.molstruc.2007.11.051
Indexed BySCI
Language英语
WOS IDWOS:000260270200018
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/63054
Collection中国科学院化学研究所
Corresponding AuthorTang, Ya-Lin
AffiliationChinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Xiu-Feng,Xie, Ling,Liu, Yang,et al. Molecular interaction and energy transfer between human serum albumin and bioactive component Aloe dihydrocoumarin[J]. JOURNAL OF MOLECULAR STRUCTURE,2008,888(1-3):145-151.
APA Zhang, Xiu-Feng,Xie, Ling,Liu, Yang,Xiang, Jun-Feng,Li, Lin,&Tang, Ya-Lin.(2008).Molecular interaction and energy transfer between human serum albumin and bioactive component Aloe dihydrocoumarin.JOURNAL OF MOLECULAR STRUCTURE,888(1-3),145-151.
MLA Zhang, Xiu-Feng,et al."Molecular interaction and energy transfer between human serum albumin and bioactive component Aloe dihydrocoumarin".JOURNAL OF MOLECULAR STRUCTURE 888.1-3(2008):145-151.
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