ICCAS OpenIR
Separation of dansylated amino acid enantiomers by chiral ligand-exchange CE with a zinc(II) L-arginine complex as the selecting system
Qi, Li1,2; Chen, Yi1,2; Xie, Mingyi1,2; Guo, Zhenpeng1,2; Wang, Xiaoyu1,2
2008-11-01
Source PublicationELECTROPHORESIS
ISSN0173-0835
Volume29Issue:20Pages:4277-4283
AbstractA facile chiral ligand-exchange capillary electrophoretic method has been explored for the enantioseparation and UV detection of dansyl-amino acids with Zn(II) L-arginine complex as a chiral selecting system. Successful enantioseparation of 17 pairs of amino acid enantiomers has been achieved with a buffer of 100 mM boric acid, 5 mM ammonium acetate, 3 mM ZnSO(4) and 6 mM L-Arg at pH 8.0, of which 10 pairs were fully resolved with resolution in between 1.59 and 4.21. This new method was shown to be applicable to the separation of some mixed pairs of amino acids and to the quantitative analysis of some real samples such as rice vinegars, with a linear range between 0.8 and 150 mu g/mL, correlation coefficient above 0.99 and recovery in between 90.1 and 112.4%. It was found that amino acids with low resistance side chain(s), low tendency to form intramolecular hydrogen bond or high tendency to form intermolecular hydrogen bonds are more easily enantioseparated than those with extra carboxyl and/or phenyl groups. By the use of the suggested buffer, the running pH should be selected at 7.4-9.0 to compromise the resolution and elution speed.
KeywordDansyl-amino Acid Enantioseparation Ligand-exchange Ce Rice Vinegar Zinc Complex
DOI10.1002/elps.200800064
Indexed BySCI
Language英语
WOS IDWOS:000261456400017
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/63022
Collection中国科学院化学研究所
Corresponding AuthorChen, Yi
Affiliation1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing 100080, Peoples R China
2.Beijing Natl Lab Mol Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Qi, Li,Chen, Yi,Xie, Mingyi,et al. Separation of dansylated amino acid enantiomers by chiral ligand-exchange CE with a zinc(II) L-arginine complex as the selecting system[J]. ELECTROPHORESIS,2008,29(20):4277-4283.
APA Qi, Li,Chen, Yi,Xie, Mingyi,Guo, Zhenpeng,&Wang, Xiaoyu.(2008).Separation of dansylated amino acid enantiomers by chiral ligand-exchange CE with a zinc(II) L-arginine complex as the selecting system.ELECTROPHORESIS,29(20),4277-4283.
MLA Qi, Li,et al."Separation of dansylated amino acid enantiomers by chiral ligand-exchange CE with a zinc(II) L-arginine complex as the selecting system".ELECTROPHORESIS 29.20(2008):4277-4283.
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