ICCAS OpenIR
MAC mode atomic force microscopy studies of living samples, ranging from cells to fresh tissue
Ge, Guanglu; Han, Dong; Lin, Danying; Chu, Weiguo; Sun, Yunxu; Jiang, Lei; Ma, Wanyun; Wang, Chen
2007-04-01
Source PublicationULTRAMICROSCOPY
ISSN0304-3991
Volume107Issue:4-5Pages:299-307
AbstractMagnetic AC mode (MAC mode) atomic force microscopy (AFM), a novel type of tapping mode AFM in which the cantilever is driven directly by a magnetic field, is a powerful tool for imaging with high spatial resolution and better signal-to-noise in liquid environment. It may largely extend the application of AFM to living samples, especially those are sensitive to cantilever forces, even to multilayer tissue samples. However, there are few reports on the imaging of living cells by MAC mode AFM previously. In our present study, we explore the optimal imaging conditions of MAC mode AFM on living astrocytes and fresh arterial intima surface. We also used nude tips for PicoTREC panel (i.e., Aux in BNC, a new data collecting channel) to image living samples and discussed its difference with phase imaging. We show that living biological samples can be imaged by MAC mode AFM at details of comparable resolution as those by high resolution scanning electron microscopy. Furthermore, the combination of height, amplitude, phase and TREC panel signals provide abundant informations for the characteristics of living samples, such as topography, profile, stiffness and adhesion. (c) 2006 Elsevier B.V. All rights reserved.
KeywordAtomic Force Microscopy Magnetic Ac Mode Astrocytes Arterial Intima Field Emission Scanning Electron Microscopy
DOI10.1016/j.ultramic.2006.08.009
Indexed BySCI
Language英语
WOS IDWOS:000244373700004
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/62918
Collection中国科学院化学研究所
Corresponding AuthorHan, Dong
Affiliation1.Natl Ctr Nanosci & Technol, Beijing 100080, Peoples R China
2.Tsinghua Univ, Dept Phys, Beijing 100084, Peoples R China
3.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Ge, Guanglu,Han, Dong,Lin, Danying,et al. MAC mode atomic force microscopy studies of living samples, ranging from cells to fresh tissue[J]. ULTRAMICROSCOPY,2007,107(4-5):299-307.
APA Ge, Guanglu.,Han, Dong.,Lin, Danying.,Chu, Weiguo.,Sun, Yunxu.,...&Wang, Chen.(2007).MAC mode atomic force microscopy studies of living samples, ranging from cells to fresh tissue.ULTRAMICROSCOPY,107(4-5),299-307.
MLA Ge, Guanglu,et al."MAC mode atomic force microscopy studies of living samples, ranging from cells to fresh tissue".ULTRAMICROSCOPY 107.4-5(2007):299-307.
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