Radioiodination and biodistribution of PEG(y) lated YP13 polypeptide
Sun Li-Yan; Chu Tai-Wi; Wang Yi; Wang Xiang-Yun
AbstractA dodeca-peptide SVSVGMLPSHAP that targets human CL-187 tumor was identified in vivo by phage display technology. In order to evaluate the potency of YP13 as the imaging agent for diagnosis of tumor and to be easily radioiodinated, peptide YSVSVGMLPSHAP(YP13) with tyramine being added to the N-terminal was synthesized and chemically modified by conjugation with a low molecular weight monomethoxy polyethylene glycol( mPEG, M-w = 5000). YP13, mPEG-YP13 and YR13 ( a nonspecific random 13 peptide as a control peptide) were labeled with I-131 via Iodogen method. The labeled complex I-131-YP13, I-131-mPEG-YP13 and I-131-YR13 were isolated and purified by RP-HPLC. The radiochemical purity was estimated to be better than 95%. The RP-HPLC analysis of the supernatant of I-131-YP13 and I-131-mPEG-YP13 in serum collected for 60 min after injection shows that I-131-mPEG-YP13 in vivo was far more stable than I-131-YP13. The biodistribution of I-131-YP13, I-131-mPEG-YP13 and the control peptide I-131-YR13 in mice bearing human colon cancinoma was investigated. The experimental results indicate that there was higher uptake of I-131-YP13 and I-131-mPEG-YP13 in tumor than that of control peptide YR13 at 1 h and 2 h. The ratios of tumor to blood for I-131-mPEG-YP13 and I-131-YP13 increased as time prolonging. The longer serum half-life of mPEG-YP13 improved the uptake in tumor. Therefore, it can be expected that the radioiodinated YP13 and PEGylated YP13 may be a potential tumor-imaging agent.
KeywordPeptide Phage Display Technology Peg I-131 Radioiodination Biodistribution
Indexed BySCI
WOS IDWOS:000245874300005
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorSun Li-Yan
AffiliationPeking Univ, Coll Chem & Mol Engn, Dept Appl Chem, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Sun Li-Yan,Chu Tai-Wi,Wang Yi,et al. Radioiodination and biodistribution of PEG(y) lated YP13 polypeptide[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2007,28(4):621-626.
APA Sun Li-Yan,Chu Tai-Wi,Wang Yi,&Wang Xiang-Yun.(2007).Radioiodination and biodistribution of PEG(y) lated YP13 polypeptide.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,28(4),621-626.
MLA Sun Li-Yan,et al."Radioiodination and biodistribution of PEG(y) lated YP13 polypeptide".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 28.4(2007):621-626.
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