ICCAS OpenIR
Demonstration of phycobilisome mobility by the time- and space-correlated fluorescence imaging of a cyanobacterial cell
Yang, Shuzhen; Su, Zuqi; Li, Heng; Feng, Juanjuan; Xie, Jie; Xia, Andong; Gong, Yandao; Zhao, Jingquan
2007
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
ISSN0005-2728
Volume1767Issue:1Pages:15-21
AbstractThe cell-wide mobility of PBSs was confirmed by synchronously monitoring the fluorescence recovery after photobleaching (FRAP) and the fluorescence loss in photobleaching (FLIP). On the other hand, a fluorescence recovery was still observed even if PBSs were immobile (PBSs fixed on the membranes by betaine and isolated PBSs fixed on the agar plate) or PBS mobility was unobservable (cell wholly bleached). Furthermore, it was proved that some artificial factors were involved not only in FRAP but also in FLIP, including renaturation of the reversibly denatured proteins, laser scanning-induced fluorescence loss and photo-damage to the cell. With consideration of the fast renaturation component in fluorescence recovery, the diffusion coefficient was estimated to be tenfold smaller than that without the component. Moreover, it was observed that the fluorescence intensity on the bleached area was always lower than that on the non-bleached area, even after 20 min, while it should be equal if PBSs were mobile freely. Based on the increasing proportion of the PBSs anti-washed to Triton X-100 (1%) with prolonged laser irradiation to the cells locked in light state 1 by PBQ, it was concluded that some PBSs became immobile due to photo-linking to PSII. (c) 2006 Elsevier B.V. All rights reserved.
KeywordMobility Phycobilisome Frap Flip Photosystem i Photosystem Ii
DOI10.1016/j.bbabio.2006.11.012
Indexed BySCI
Language英语
WOS IDWOS:000243983500002
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/62884
Collection中国科学院化学研究所
Corresponding AuthorZhao, Jingquan
Affiliation1.Chinese Acad Sci, Inst Chem, Photochem Lab, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
2.Tsing Hua Univ, Dept Biol Sci & Biotechnol, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
Yang, Shuzhen,Su, Zuqi,Li, Heng,et al. Demonstration of phycobilisome mobility by the time- and space-correlated fluorescence imaging of a cyanobacterial cell[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2007,1767(1):15-21.
APA Yang, Shuzhen.,Su, Zuqi.,Li, Heng.,Feng, Juanjuan.,Xie, Jie.,...&Zhao, Jingquan.(2007).Demonstration of phycobilisome mobility by the time- and space-correlated fluorescence imaging of a cyanobacterial cell.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1767(1),15-21.
MLA Yang, Shuzhen,et al."Demonstration of phycobilisome mobility by the time- and space-correlated fluorescence imaging of a cyanobacterial cell".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1767.1(2007):15-21.
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