ICCAS OpenIR
Chiral CE of aromatic amino acids by ligand-exchange with zinc(II)-L-lysine complex
Qi, Li; Han, Yanli; Zuo, Min; Chen, Yi
2007-08-01
Source PublicationELECTROPHORESIS
ISSN0173-0835
Volume28Issue:15Pages:2629-2634
AbstractA novel method of chiral ligand-exchange CE was developed with either L- or D-lysine (Lys) as a chiral ligand and zinc(II) as a central ion. This type of chiral complexes was explored for the first time to efficiently separate either individual pairs of or mixed aromatic amino acid enantiomers. Using a running buffer of 5 mM ammonium acetate, 100 mM boric acid, 3 mM ZnSO4 center dot 7H(2)O and 6 MM L-Lys at pH 7.6, unlabeled D,L-tryptophan, D,L-phenylalanine, and D,L-tyrosine were well separated, giving a chiral resolution of up to 7.09. The best separation was obtained at a Lys-to-zinc ratio of 2:1, zinc concentration of 2-4 mM and running buffer pH 7.6. The buffer pH was determined to have a strong influence on resolution, while buffer composition and concentration impacted on both the resolution and peak shape. Boric acid with some ammonium acetate was an adoptable buffer system, and some additives like ethylene diamine tetraacetic acid capable of destroying the complex should be avoided. Fine-tuning of the chiral resolution and elution order was achieved by regulating the ratio Of L-Lys to D-Lys; i.e. the resolution increased from zero to its highest value as the ratio ascended from 1:0 to 1:infinitive, and L-isomers eluted before or after D-isomers in excessive D- or L-Lys, respectively.
KeywordAromatic Amino Acid Enantioseparation Ligand-exchange Ce Zinc-l-lys Complex
DOI10.1002/elps.200600740
Indexed BySCI
Language英语
WOS IDWOS:000248938400011
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:41[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/62778
Collection中国科学院化学研究所
Corresponding AuthorChen, Yi
AffiliationChinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Qi, Li,Han, Yanli,Zuo, Min,et al. Chiral CE of aromatic amino acids by ligand-exchange with zinc(II)-L-lysine complex[J]. ELECTROPHORESIS,2007,28(15):2629-2634.
APA Qi, Li,Han, Yanli,Zuo, Min,&Chen, Yi.(2007).Chiral CE of aromatic amino acids by ligand-exchange with zinc(II)-L-lysine complex.ELECTROPHORESIS,28(15),2629-2634.
MLA Qi, Li,et al."Chiral CE of aromatic amino acids by ligand-exchange with zinc(II)-L-lysine complex".ELECTROPHORESIS 28.15(2007):2629-2634.
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