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Spectroscopic investigation on the interaction of J-aggregate with human serum albumin
Zhang, Yazhou; Du, Hongyan; Tang, Yalin; Xu, Guangzhi; Yan, Wenpeng
2007-07-01
Source PublicationBIOPHYSICAL CHEMISTRY
ISSN0301-4622
Volume128Issue:2-3Pages:197-203
AbstractThe interactions of three cyanine dyes, which exhibit different meso substituent in polymethine chain, with human serum albumin (HSA) have been investigated by the means of absorption, fluorescence and circular dichroism (CD) spectra. In phosphate buffer solution (PBS), the mentioned dyes exist not as isolated monomers but rather in the formation of J-aggregation. In the presence of HSA, the absorption and fluorescence emission spectra indicated that the J-aggregation was decomposed to monomer because of the strong affinity between dye molecules and HSA. Besides the association of cyanine dyes with HSA, binding to HSA gave rise to the J-aggregation CD signals. The meso substituent in the polymethine plays an important role in the interaction of HSA and the J-aggregation. Spectral studies showed that the dye bound with HSA in a 1:1 formation. The apparent constant (K.) value was roughly identified by analysis of the corresponding fluorescence data at various HSA concentrations. The higher affinity of the molecule with meso phenyl towards HSA with respect to molecules with meso ethyl or methyl can be attributed to the arrangement of molecules in J-aggregation and the hydrophobic force between the molecules and HSA. (c) 2007 Elsevier B.V. All rights reserved.
KeywordHuman Serum Albumin J-aggregation Interaction Induced Cd Apparent Constant
DOI10.1016/j.bpc.2007.04.002
Indexed BySCI
Language英语
WOS IDWOS:000247582800011
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/62770
Collection中国科学院化学研究所
Corresponding AuthorTang, Yalin
Affiliation1.Chinese Acad Sci, Inst Chem, BNLMS, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
3.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yazhou,Du, Hongyan,Tang, Yalin,et al. Spectroscopic investigation on the interaction of J-aggregate with human serum albumin[J]. BIOPHYSICAL CHEMISTRY,2007,128(2-3):197-203.
APA Zhang, Yazhou,Du, Hongyan,Tang, Yalin,Xu, Guangzhi,&Yan, Wenpeng.(2007).Spectroscopic investigation on the interaction of J-aggregate with human serum albumin.BIOPHYSICAL CHEMISTRY,128(2-3),197-203.
MLA Zhang, Yazhou,et al."Spectroscopic investigation on the interaction of J-aggregate with human serum albumin".BIOPHYSICAL CHEMISTRY 128.2-3(2007):197-203.
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