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Hemoglobin protein hollow shells fabricated through covalent layer-by-layer technique
Duan, Li; He, Qiang; Yan, Xuehai; Cui, Yue; Wang, Kewei; Li, Junbai
2007-03-09
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume354Issue:2Pages:357-362
AbstractHemoglobin (Hb) protein microcapsules held together by cross-linker, glutaraldehyde (GA), were successfully fabricated by covalent layer-by-laver (LbL) technique. The Schiff base reaction occurred on the colloid templates between the aldehyde groups of GA and free amino sites of Hb results in the formation of GA/Hb microcapsules after the removal of the templates. The structure of obtained monodisperse protein microcapsule was characterized by transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). The UV-Vis spectra measurements demonstrate the existence of Hb in the assembled capsules. Cyclic voltammetry (CV) and potential-controlled amperometric measurements (I-t curve) confirm that hemoglobin microcapsules after fabrication remain their heme electroactivity. Moreover, direct electron transfer process from protein to electrode surface was performed to detect the heme electrochemistry without using any mediator or promoter. The experiments of fluorescence recovery after photobleaching (FRAP) by CLSM demonstrate that the hemoglobin protein microcapsules have an improved permeability comparing to the conventional polyelectrolyte microcapsules. (c) 2006 Elsevier Inc. All rights reserved.
KeywordMicrocapsules Hemoglobin Protein Glutaraldehyde Layer-by-layer Assembly
DOI10.1016/j.bbrc.2006.12.223
Indexed BySCI
Language英语
WOS IDWOS:000244050000006
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Citation statistics
Cited Times:75[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/62610
Collection中国科学院化学研究所
Corresponding AuthorLi, Junbai
Affiliation1.Chinese Acad Sci, Inst Chem, CAS Key Lab Colloid & Interface Sci, BNLMS,Int Joint Lab, Beijing 100080, Peoples R China
2.Max Planck Inst Colloids & Interfaces, D-14476 Potsdam, Germany
Recommended Citation
GB/T 7714
Duan, Li,He, Qiang,Yan, Xuehai,et al. Hemoglobin protein hollow shells fabricated through covalent layer-by-layer technique[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,354(2):357-362.
APA Duan, Li,He, Qiang,Yan, Xuehai,Cui, Yue,Wang, Kewei,&Li, Junbai.(2007).Hemoglobin protein hollow shells fabricated through covalent layer-by-layer technique.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,354(2),357-362.
MLA Duan, Li,et al."Hemoglobin protein hollow shells fabricated through covalent layer-by-layer technique".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 354.2(2007):357-362.
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