ICCAS OpenIR
Synthesis of alpha-chymotrypsin/polymer composites by a reverse micelle/gas antisolvent method
Li, Wei; Zhang, Hanling; Zhang, Chaoxing; Feng, Xiaoying; Han, Buxing; Yang, Guanying
2007-09-01
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume59Issue:1Pages:11-15
Abstractalpha-Chymotrypsin (CT)/polyvinylpyrrolidone (PVP) composites was synthesized by combination of reverse micelles and CO2. In this method, the two reverse micellar solutions containing CT and PVP, respectively, were first mixed, then compressed CO2 was used as an antisolvent to precipitate the CT and PVP simultaneously and CT/PVP composites were successfully prepared. The morphology of the obtained CT/PVP composites was characterized by transmission electron microscopy (TEM). The FTIR spectra of the composites showed that there was interaction between CT and PVP. The storage activity of the enzyme immobilized on the polymer by this method was higher than that of the pure enzyme. This method has some advantages and can be easily applied to the synthesis of some other enzyme/polymer composites. (C) 2007 Elsevier B.V. All rights reserved.
KeywordAlpha-chymotripsin Polyvinylpyrrolidone Reverse Micelle Compressed Co2
DOI10.1016/j.colsurfb.2007.04.011
Indexed BySCI
Language英语
WOS IDWOS:000248642900002
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/62570
Collection中国科学院化学研究所
Corresponding AuthorHan, Buxing
AffiliationChinese Acad Sci, Ctr Mol Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Li, Wei,Zhang, Hanling,Zhang, Chaoxing,et al. Synthesis of alpha-chymotrypsin/polymer composites by a reverse micelle/gas antisolvent method[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2007,59(1):11-15.
APA Li, Wei,Zhang, Hanling,Zhang, Chaoxing,Feng, Xiaoying,Han, Buxing,&Yang, Guanying.(2007).Synthesis of alpha-chymotrypsin/polymer composites by a reverse micelle/gas antisolvent method.COLLOIDS AND SURFACES B-BIOINTERFACES,59(1),11-15.
MLA Li, Wei,et al."Synthesis of alpha-chymotrypsin/polymer composites by a reverse micelle/gas antisolvent method".COLLOIDS AND SURFACES B-BIOINTERFACES 59.1(2007):11-15.
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