ICCAS OpenIR
Selective protein immobilization via DNA conjugation under microfluidic laminar flow
Guo Su1; Xue Mian-Qi2; Qian Min-Xie1; Cao Ting-Bing2; Zhao Xin-Sheng1
2007-12-01
Source PublicationACTA PHYSICO-CHIMICA SINICA
ISSN1000-6818
Volume23Issue:12Pages:1827-1830
AbstractThe feasibility of controlled protein immobilization via DNA conjugation by utilizing laminar flow in a microfluidic device was demonstrated. The glass surface in a microchannel was treated by oligonucleotides. The laminar flow brought different protein-DNA conjugates parallel into the microchannel. DNA recognition allows proteins to be delivered to the desired location. The total internal reflection fluorescence was also applied to monitor the process. Both the specificity and sensitivity were high, and the immobilization and removal of the proteins were repeatable. It was shown that with parallel detection of specific and non-specific recognitions, the accuracy of bioassay would be effectively enhanced. This strategy could improve the performing quality of biosensors in microfluidic devices.
KeywordMicrofluidic Device Proteinsensor Dna-directed Immobilization Total Internal Reflection Fluorescence
Indexed BySCI
Language英语
WOS IDWOS:000251812900001
PublisherPEKING UNIV PRESS
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/62434
Collection中国科学院化学研究所
Corresponding AuthorZhao Xin-Sheng
Affiliation1.Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, Beijing Natl Lab Mol Sci,State Key Lab Struct Che, Beijing 100871, Peoples R China
2.Renmin Unin China, Dept Chem, Beijing 100872, Peoples R China
Recommended Citation
GB/T 7714
Guo Su,Xue Mian-Qi,Qian Min-Xie,et al. Selective protein immobilization via DNA conjugation under microfluidic laminar flow[J]. ACTA PHYSICO-CHIMICA SINICA,2007,23(12):1827-1830.
APA Guo Su,Xue Mian-Qi,Qian Min-Xie,Cao Ting-Bing,&Zhao Xin-Sheng.(2007).Selective protein immobilization via DNA conjugation under microfluidic laminar flow.ACTA PHYSICO-CHIMICA SINICA,23(12),1827-1830.
MLA Guo Su,et al."Selective protein immobilization via DNA conjugation under microfluidic laminar flow".ACTA PHYSICO-CHIMICA SINICA 23.12(2007):1827-1830.
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