ICCAS OpenIR
Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study
Zhao, Liping1,4; Qiao, Juan1,2; Zhang, Ke2,3; Li, Dan5; Zhang, Hongyi4; Qi, Li1,2
2018-05-04
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1548Pages:104-110
AbstractA chiral ligand exchange capillary electrochromatography (CLE-CEC) protocol was designed and implemented for D,L-amino acids enantioseparation with poly(maleic anhydride-styrene-methacryloylL-arginine methyl ester) as the coating. The block copolymer was synthesized through the reversible addition fragmentation chain transfer reaction. In the constructed CLE-CEC system, poly (methacryloylL-arginine methyl ester) moiety of the block copolymer played the role as the immobilized chiral ligand and Zn (II) was used as the central ion. Key factors, including pH of buffer solution, ratio of Zn (II) to ligands, the mass ratio of monomers in the block copolymer, which affect the enantioresolution were investigated. Comparing with the bare capillary, the CLE-CEC enantioresolution was enhanced greatly with the coating one. 5 Pairs of D,L-amino acids enantiomers obtained baseline separation with 5 pairs partly separated. The mechanism of enhancement enantioresolution of the developed CLE-CEC system was explored briefly. Further, good linearities were achieved in the range of 25.0 mu M-5.0 mM for quantitative analysis of D-glutamine (r(2) = 0.997) and L-glutamine (r(2) = 0.991). Moreover, the proposed CLE-CEC assay was successfully applied in the kinetics study of glutaminase by using L-glutamine as the substrate. (C) 2018 Elsevier B.V. All rights reserved.
KeywordChiral Ligand Exchange Capillary Electrochromatography Enantioseparation Block Copolymer Coating Enzyme Kinetics Study
DOI10.1016/j.chroma.2018.03.031
Indexed BySCI
Language英语
WOS IDWOS:000430779500013
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/43132
Collection中国科学院化学研究所
Corresponding AuthorZhang, Hongyi; Qi, Li
Affiliation1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, 2 Zhongguancun Beiyijie, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, 2 Zhongguancun Beiyijie, Beijing 100190, Peoples R China
4.Hebei Univ, Key Lab Analyt Sci & Technol Hebei Prov, Coll Chem & Environm Sci, 180 Wusi East Rd, Baoding 071002, Peoples R China
5.Jinan Univ, Guangzhou 510632, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Liping,Qiao, Juan,Zhang, Ke,et al. Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1548:104-110.
APA Zhao, Liping,Qiao, Juan,Zhang, Ke,Li, Dan,Zhang, Hongyi,&Qi, Li.(2018).Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study.JOURNAL OF CHROMATOGRAPHY A,1548,104-110.
MLA Zhao, Liping,et al."Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study".JOURNAL OF CHROMATOGRAPHY A 1548(2018):104-110.
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