Bioinspired Peptide for Imaging Hg2+ Distribution in Living Cells and Zebrafish Based on Coordination-Mediated Supramolecular Assembling

doi:10.1021/acs.analchem.8b00059

ICCAS OpenIR

	Bioinspired Peptide for Imaging Hg2+ Distribution in Living Cells and Zebrafish Based on Coordination-Mediated Supramolecular Assembling
	Gui, Shilang 1,2; Huang, Yanyan 1,2; Hu, Fang 1,2; Jin, Yulong 1,2; Zhang, Guanxin 1,2; Zhang, Deqing 1,2; Zhao, Rui 1,2
	2018-08-21
Source Publication	ANALYTICAL CHEMISTRY
ISSN	0003-2700
Volume	90 Issue:16 Pages:9708-9715
Abstract	Peptides with modular structure provide a tailorable platform for constructing responsive supramolecular assemblies, which are attractive as functional biomaterials and smart sensors. In this work, the feasibility of regulating small peptides assembly with molecular design and metal ion recognition was demonstrated. Tripeptides were designed and found to have diverse response and self-assembly behavior to Hg2+. The incorporation of an aggregation-induced emission fluorophore TPE enabled the visualization of Hg2+ recognition and the assembly phenomenon. A structural analogue (Pep2) to gamma-glutathione was identified with high specificity and nanomolar response to Hg2+ both in buffer solution and living cells. Driven by the coordination force and noncovalent intramolecular stacking, assembling of twisted nanofibers from Pep2-TPE and Hg2+ were observed. Benefiting from its biocompatibility, fast and switchable fluorescence response, Pep2-TPE was applied for imaging and monitoring Hg2+ distribution in living cells and zebrafish. With good permeability to plasma membrane and tissues, Pep2-TPE indicated the preferential distribution of Hg2+ in cell nucleoli and brain of zebrafish, which is related with the deleterious effect of inorganic mercury in living biosystems.
DOI	10.1021/acs.analchem.8b00059
Indexed By	SCI
Language	英语
WOS ID	WOS:000442707100011
Publisher	AMER CHEMICAL SOC
Citation statistics	Cited Times:6[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.iccas.ac.cn/handle/121111/42356
Collection	中国科学院化学研究所
Corresponding Author	Huang, Yanyan; Zhao, Rui
Affiliation	1.Chinese Acad Sci, Beijing Natl Res Ctr Mol Sci, CAS Key Labs Analyt Chem Living Biosyst & Organ S, Inst Chem,CAS Res Educ Ctr Excellence Mol Sci, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation GB/T 7714	Gui, Shilang,Huang, Yanyan,Hu, Fang,et al. Bioinspired Peptide for Imaging Hg2+ Distribution in Living Cells and Zebrafish Based on Coordination-Mediated Supramolecular Assembling[J]. ANALYTICAL CHEMISTRY,2018,90(16):9708-9715.
APA	Gui, Shilang.,Huang, Yanyan.,Hu, Fang.,Jin, Yulong.,Zhang, Guanxin.,...&Zhao, Rui.(2018).Bioinspired Peptide for Imaging Hg2+ Distribution in Living Cells and Zebrafish Based on Coordination-Mediated Supramolecular Assembling.ANALYTICAL CHEMISTRY,90(16),9708-9715.
MLA	Gui, Shilang,et al."Bioinspired Peptide for Imaging Hg2+ Distribution in Living Cells and Zebrafish Based on Coordination-Mediated Supramolecular Assembling".ANALYTICAL CHEMISTRY 90.16(2018):9708-9715.

Files in This Item:
There are no files associated with this item.

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Institution:
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh

中国科学院化学研究所机构知识库