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Bioinspired Peptide for Imaging Hg2+ Distribution in Living Cells and Zebrafish Based on Coordination-Mediated Supramolecular Assembling
Gui, Shilang1,2; Huang, Yanyan1,2; Hu, Fang1,2; Jin, Yulong1,2; Zhang, Guanxin1,2; Zhang, Deqing1,2; Zhao, Rui1,2
2018-08-21
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume90Issue:16Pages:9708-9715
AbstractPeptides with modular structure provide a tailorable platform for constructing responsive supramolecular assemblies, which are attractive as functional biomaterials and smart sensors. In this work, the feasibility of regulating small peptides assembly with molecular design and metal ion recognition was demonstrated. Tripeptides were designed and found to have diverse response and self-assembly behavior to Hg2+. The incorporation of an aggregation-induced emission fluorophore TPE enabled the visualization of Hg2+ recognition and the assembly phenomenon. A structural analogue (Pep2) to gamma-glutathione was identified with high specificity and nanomolar response to Hg2+ both in buffer solution and living cells. Driven by the coordination force and noncovalent intramolecular stacking, assembling of twisted nanofibers from Pep2-TPE and Hg2+ were observed. Benefiting from its biocompatibility, fast and switchable fluorescence response, Pep2-TPE was applied for imaging and monitoring Hg2+ distribution in living cells and zebrafish. With good permeability to plasma membrane and tissues, Pep2-TPE indicated the preferential distribution of Hg2+ in cell nucleoli and brain of zebrafish, which is related with the deleterious effect of inorganic mercury in living biosystems.
DOI10.1021/acs.analchem.8b00059
Indexed BySCI
Language英语
WOS IDWOS:000442707100011
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/42356
Collection中国科学院化学研究所
Corresponding AuthorHuang, Yanyan; Zhao, Rui
Affiliation1.Chinese Acad Sci, Beijing Natl Res Ctr Mol Sci, CAS Key Labs Analyt Chem Living Biosyst & Organ S, Inst Chem,CAS Res Educ Ctr Excellence Mol Sci, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Gui, Shilang,Huang, Yanyan,Hu, Fang,et al. Bioinspired Peptide for Imaging Hg2+ Distribution in Living Cells and Zebrafish Based on Coordination-Mediated Supramolecular Assembling[J]. ANALYTICAL CHEMISTRY,2018,90(16):9708-9715.
APA Gui, Shilang.,Huang, Yanyan.,Hu, Fang.,Jin, Yulong.,Zhang, Guanxin.,...&Zhao, Rui.(2018).Bioinspired Peptide for Imaging Hg2+ Distribution in Living Cells and Zebrafish Based on Coordination-Mediated Supramolecular Assembling.ANALYTICAL CHEMISTRY,90(16),9708-9715.
MLA Gui, Shilang,et al."Bioinspired Peptide for Imaging Hg2+ Distribution in Living Cells and Zebrafish Based on Coordination-Mediated Supramolecular Assembling".ANALYTICAL CHEMISTRY 90.16(2018):9708-9715.
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