ICCAS OpenIR  > 分子动态与稳态结构实验室
Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe
Zhang, Suge1,2; Sun, Hongxia1; Wang, Lixia1; Liu, Yan1; Chen, Hongbo1,2; Li, Qian1; Guan, Aijiao1; Liu, Meirong3; Tang, Yalin1,2
2018-09-06
Source PublicationNUCLEIC ACIDS RESEARCH
ISSN0305-1048
Volume46Issue:15Pages:7522-7532
AbstractG-quadruplex DNA has been viewed as a prospective anti-cancer target owing to its potential biological relevance. Real-time monitoring of DNA G-quadruplex structures in living cells can provide valuable insights into the relationship between G-quadruplex formation and its cellular consequences. However, the probes capable of detecting DNA G-quadruplexes in living cells are still very limited. Herein, we reported a new fluorescent probe, IMT, for real-time visualization of DNA G-quadruplex structures in living cells. Using IMT as a fluorescent indicator, the quantity changes of DNA G-quadruplex at different points in time during continuous cellular progression responding to Aphidicolin and Hydroxyurea treatment have been directly visualized. Our data demonstrate that IMT will be a valuable tool for exploring DNA G-quadruplexes in live cells. Further application of IMT in fluorescence imaging may reveal more information on the roles of DNA G-quadruplexes in biological systems.
DOI10.1093/nar/gky665
Indexed BySCI
Language英语
WOS IDWOS:000444148100011
PublisherOXFORD UNIV PRESS
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/41890
Collection分子动态与稳态结构实验室
Corresponding AuthorSun, Hongxia; Tang, Yalin
Affiliation1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, CAS Res Educ Ctr Excellence Mol Sci,Inst Chem, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Ctr Physiochem Anal & Measurement, Inst Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Suge,Sun, Hongxia,Wang, Lixia,et al. Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe[J]. NUCLEIC ACIDS RESEARCH,2018,46(15):7522-7532.
APA Zhang, Suge.,Sun, Hongxia.,Wang, Lixia.,Liu, Yan.,Chen, Hongbo.,...&Tang, Yalin.(2018).Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe.NUCLEIC ACIDS RESEARCH,46(15),7522-7532.
MLA Zhang, Suge,et al."Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe".NUCLEIC ACIDS RESEARCH 46.15(2018):7522-7532.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Suge]'s Articles
[Sun, Hongxia]'s Articles
[Wang, Lixia]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Suge]'s Articles
[Sun, Hongxia]'s Articles
[Wang, Lixia]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Suge]'s Articles
[Sun, Hongxia]'s Articles
[Wang, Lixia]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.