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题名: An intramolecular charge transfer process based fluorescent probe for monitoring subtle pH fluctuation in living cells
作者: Sun, Mingtai1, 2; Du, Libo3; Yu, Huan2; Zhang, Kui2; Liu, Yang3; Wang, Suhua1, 2
关键词: pH sensor ; Fluorescent probe ; Benzothiazole derivative ; Living cell imaging ; Intramolecular charge transfer ; Ratiometric
刊名: TALANTA
发表日期: 2017
卷: 162, 页:180-186
收录类别: SCI
英文摘要: It is crucial to monitor intracellular pH values and their fluctuation since the organelles of cells have different pH distribution. Herein we construct a new small molecule fluorescent probe HBT-O for monitoring the subtle pH values within the scope of neutral to acid in living cells. The probe exhibited good water solubility, a marked turquoise to olivine emission color change in response to pH, and tremendous fluorescence hypochromatic shift of similar to 50 nm (1718 cm(-1)) as well as the increased fluorescence intensity when the pH value changed from neutral to acid. Thus, the probe HBT-O can distinguish the subtle changes in the range of normal pH values from neutral to acid with significant fluorescence changes. These properties can be attributed to the intramolecular charge transfer (ICT) process of the probe upon protonation in buffer solutions at varied pH values. Moreover, the probe was reversible and nearly non-toxic for living cells. Then the probe was successfully used to detect pH fluctuation in living cells by exhibiting different fluorescence colors and intensity. These findings demonstrate that the probe will find useful applications in biology and biomedical research.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/36527
Appears in Collections:分子动态与稳态结构实验室_期刊论文

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作者单位: 1.North China Elect Power Univ, Sch Environm & Chem Engn, Beijing 102206, Peoples R China
2.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
3.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China
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