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题名: Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots
作者: Wang, Yashan1; Ma, Tiancong2; Ma, Shuyue3; Liu, Yongjun1; Tian, Yaping1; Wang, Ruinan1; Jiang, Yanbin1; Hou, Dongjun1; Wang, Jianlong3
关键词: Veterinary antibiotic ; Aptasensor ; Molybdenum disulfide MoS2 ; Exfoliation ; Fluorescence resonance energy transfer (FRET) ; Milk analysis
刊名: MICROCHIMICA ACTA
发表日期: 2017
卷: 184, 期:1, 页:203-210
收录类别: SCI
英文摘要: A rapid and sensitive aptamer-based assay is described for kanamycin, a veterinary antibiotic with neurotoxic side effects. It is based on a novel FRET pair consisting of fluorescent carbon dots and layered MoS2. This donor-acceptor pair (operated at excitation/emission wavelengths of 380/440 nm) shows fluorescence recovery efficiencies reaching 93 %. By taking advantages of aptamer-induced fluorescence quenching and recovery, kanamycin can be quantified in the of 4-25 mu M concentration range, with a detection limit of 1.1 mu M. The method displays good specificity and was applied to the determination of kanamycin in spiked milk where it gave recoveries ranging from 85 % to 102 %, demonstrating that the method serves as a promising tool for the rapid detection of kanamycin in milk and other animal-derived foodstuff.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/36431
Appears in Collections:其它_期刊论文

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作者单位: 1.China Anim Dis Control Ctr, Minist Agr, 20 Maizidian St, Beijing 100125, Peoples R China
2.Chinese Acad Sci, Inst Chem, Bei Yi Jie 2, Beijing 100190, Peoples R China
3.Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China
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